利用CRISPR/Cas9技术研究玉米ZmFKF1在开花过程中的作用
杨敏1(
),胥华伟2,王翠玲2,杨护1,魏岳荣1()
),胥华伟2,王翠玲2,杨护1,魏岳荣1()
Using CRISPR/Cas9-mediated Targeted Mutagenesis of ZmFKF1 Delayed Flowering Time in Maize
YANG Min1(),XU HuaWei2,WANG CuiLing2,YANG Hu1,WEI YueRong1()
),XU HuaWei2,WANG CuiLing2,YANG Hu1,WEI YueRong1()
图5. ZmFKF1编辑植株的表型分析及关键基因的表达情况
A：C7-1和B104在播种后67 d的表型;B：C7-1和B104在播种后70 d的表型;C：不同基因型材料中开花关键基因的表达情况,其中ZmUbi为内参基因,利用2-ΔΔCT方法计算基因相对表达量结果
Fig. 5. Phenotype and flowering related genes expression analysis of ZmFKF1 editing lines
A: The phenotype of C7-1 compared to ‘B104’ at 67 days after sowing; B: The phenotype of C7-1 compared to ‘B104’ at 70 days after sowing; C: Expression analysis of flowering related genes in different genotype lines. ZmUbi primers were used for PCR as an internal control, the result of relative genes expression was calculated by 2-ΔΔCT method
