利用CRISPR/Cas9技术研究玉米ZmFKF1在开花过程中的作用
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杨敏 1(  ),胥华伟 2,王翠玲 2,杨护 1,魏岳荣 1(  )
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Using CRISPR/Cas9-mediated Targeted Mutagenesis of ZmFKF1 Delayed Flowering Time in Maize
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YANG Min 1(  ),XU HuaWei 2,WANG CuiLing 2,YANG Hu 1,WEI YueRong 1(  )
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图5. ZmFKF1编辑植株的表型分析及关键基因的表达情况 A:C7-1和B104在播种后67 d的表型;B:C7-1和B104在播种后70 d的表型;C:不同基因型材料中开花关键基因的表达情况,其中ZmUbi为内参基因,利用2-ΔΔCT方法计算基因相对表达量结果
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Fig. 5. Phenotype and flowering related genes expression analysis of ZmFKF1 editing lines A: The phenotype of C7-1 compared to ‘B104’ at 67 days after sowing; B: The phenotype of C7-1 compared to ‘B104’ at 70 days after sowing; C: Expression analysis of flowering related genes in different genotype lines. ZmUbi primers were used for PCR as an internal control, the result of relative genes expression was calculated by 2-ΔΔCT method
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