利用CRISPR/Cas9技术研究玉米ZmFKF1在开花过程中的作用
杨敏1(
),胥华伟2,王翠玲2,杨护1,魏岳荣1()
),胥华伟2,王翠玲2,杨护1,魏岳荣1()
Using CRISPR/Cas9-mediated Targeted Mutagenesis of ZmFKF1 Delayed Flowering Time in Maize
YANG Min1(),XU HuaWei2,WANG CuiLing2,YANG Hu1,WEI YueRong1()
),XU HuaWei2,WANG CuiLing2,YANG Hu1,WEI YueRong1()
图1. gRNA靶位点、FKF1分子进化树分析及pYLCRISPR/Cas9-ZmFKF1-T1质粒的T-DNA区示意图
A：靶位点在ZmFKF1中的位置;B：9个物种的FKF1分子进化树分析;C：pYLCRISPR/Cas9-ZmFKF1-T1质粒T-DNA区示意图
Fig. 1. Target site of the gRNA in the ZmFKF1 gene, phylogenetic tree analysis of FKF1 amino acid sequences in nine species and the diagram of T-DNA region of pYLCRISPR/Cas9-ZmFKF1-T1 plasmid
A: Position of the target in ZmFKF1 gene locus; B: Phylogenetic tree analysis of FKF1 amino acid sequences in 9 species; C: The diagram of T-DNA region of pYLCRISPR/Cas9-ZmFKF1-T1 plasmid
