243份云南普通小麦地方品种抗条锈病鉴定及分子标记检测

习玲,王昱琦,杨修,朱微,陈国跃,王益,覃鹏,周永红,康厚扬

Evaluation of Resistance to Stripe Rust and Molecular Detection of Resistance Gene(s) in 243 Common Wheat Landraces from the Yunnan Province

XI Ling,WANG YuQi,YANG Xiu,ZHU Wei,CHEN GuoYue,WANG Yi,QIN Peng,ZHOU YongHong,KANG HouYang

表1 抗条锈病基因的分子标记及引物序列

Table 1 Molecular markers and primer sequences for stripe rust resistance genes

Yr基因 Yr gene 类型 Type 分子标记 Molecular marker 引物序列 Primer sequence (5′-3′) 文献Reference Yr5 DM Yr5_B GGGAACACTTCACGATCA [31] AATTCCTTCATGCCTTCC Yr5_A CGCTTAATTCCCCTTCCTTC TGGCTCCTTATTCGTTCTCTTTC KASP Yr5F GAAGGTGACCAAGTTCATGCTGCGCCCCTTTTCGAAAAAATA Yr5H GAAGGTCGGAGTCAACGGATTCTAGCATCAAACAAGCTAAATA Yr5R ATGTCGAAATATTGCATAACATGG Yr10 AFLP SC200 CTGCAGAGTGACATCATACA [32] TCGAACTAGTAGATGCTGGC DM Yr10-5 GGAAATGTGGCGGAGTACCA [16] CGGAAGGGAGAACCACTGTC Yr10-6 CAGCTTGACAAGGGCGAGTA CGCTTGTCGCCAATTCCAAA Yr15 DM Y15K1_F2 GGAGATAGAGCACATTACAGAC [33] uhw301R GGAGATAGAGCACATTACAGAC WJL2F CCTTGTGTGCTACCAGGGTT [34] WJL2R GGAACTCAAGCCCTTCTGCT WJL3F AAAAGAGCTCGCCTCCTACG [35] WJL3R GCCATGATGAGATCGGGAGG Yr18 STS csLV34 GTTGGTTAAGACTGGTGATGG [17] TGCTTGCTATTGCTGAATAGT Cssfr1 L34DINT9F TTGATGAAACCAGTTTTTTTTCTA L34PLUSR GCCATTTAACATAATCATGATGGA Yr26 STS Xwe173 GGGACAAGGGGAGTTGAAGC [36] GAGAGTTCCAAGCAGAACAC Yr28 InDel ZCZp343F TGTGTCATGTTTGGTCGATAGG [37] ZCZp342R TCCTCCCTTGTAGCTTCACG DM P175 GCACCGTCCTTCATCTCAGT P176 TGCTTTTCCCCGTATCCCTT Yr29 CAPS csLV46 CGAGACGTCGTCTTCTCTAAC [38] GTGTATGTGTTGATTCTCCTCG Yr30 SSR Xgwm533 GTTGCTTTAGGGGAAAAGCC [39] AAGGCGAATCAAACGGAATA Yr36 DM WKS1_150F ATGGAGCTCCCACGAAACAAAC [40] WKS1_620R ACCTCCATGTTGCTCGCATTTGCT WKS1_182F TAGCTCTTCGTGGCCAAAGG WKS1_150R TGGCCACGAAGAGCTAAAGG Yr39 SSR Xgwm131 AATCCCCACCGATTCTTCTC [41] AGTTCGTGGGTCTCTGATGG Yr41 SSR Xgwm410 GCTTGAGACCGGCACAGT [42] CGAGACCTTGAGGGTCTAGA SSR Xgwm374 ATAGTGTGTTGCATGCTGTGTG TCTAATTAGCGTTGGCTGCC Yr48 SSR Xwmc727 CATAATCAGGACAGCCGCAC [43] TAGTGGCCTGATGTATCTAGTTGG SSR Xwms291 CATCCCTACGCCACTCTGC AATGGTATCTATTCCGACCCG Yr65 SSR Xgwm18 GGTTGCTGAAGAACCTTATTTAGG [19] TGGCGCCATGATTGCATTATCTTC Yr67 SSR Xbarc182 CCATGGCCAACAGCTCAAGGTCTC [44] CGCAAAACCGCATCAGGGAAGCACCAAT SSR Xcfa2040 TCAAATGATTTCAGGTAACCACTA TTCCTGATCCCACCAAACAT Yr80 KASP KASP_53113 GAAGGTGACCAAGTTCATGCTTGTACAATGACTCCTCGACTAACA [20] GAAGGTCGGAGTCAACGGATTTGTACAATGACTCCTCGACTAACG GCCACGCAATATCACCATCG Yr81 KASP KASP_3077 GAAGGTGACCAAGTTCATGCTATTCCAAAGTAATTGGCAACAGGTTCA [21] GAAGGTCGGAGTCAACGGATTCCAAAGTAATTGGCAACAGGTTCG TGTGGAGCGTGACAATGAGGAAGTT