川西北高原牦牛和藏猪源大肠杆菌生物被膜表型、耐药基因、整合酶基因和毒力基因检测

陈朝喜,李宇涵,谭敏,汪露,黄志宏

Biofilm-Forming Phenotype, Antibacterial Resistance Genes, Integrase Genes and Virulence Genes Detection of Escherichia coli Isolated from Yaks and Tibetan Pigs in Northwest Sichuan Plateau

CHEN ChaoXi,LI YuHan,TAN Min,WANG Lu,HUANG ZhiHong

表2 毒力基因扩增引物信息

Table 2 Information of PCR primers for virluence genes amplification

毒力基因类别 Types of virluence genes	基因名称 Gene name	引物序列（5′→3′） Primer sequence(5′→3′)	扩增片段大小 Amplification size(bp)	退火温度 Annealing temperature (℃)	参考文献或序列号 Reference or sequence number
产志贺毒素毒力基因 Shiga toxin-producing virulence gene	stx1	F-ACACTGGATGATCTCAGTGG	614	58	[29]
	stx1	R-CTGAATCCCCCTCCATTATG	614	58	[29]
	stx2	F-CCTGTCAACTGAGCACTTTG	779	58	[29]
	stx2	R-CCATGACAACGGACAGCAGTT	779	58	[29]
非菌毛黏附素 Non-fimbrial adhesin	eaeA	F-ATGCTTAGTGCTGGTTTAGG	248	55	EF079676
非菌毛黏附素 Non-fimbrial adhesin	eaeA	R-GCCTTCATCATTTCGCTTTC	248	55	EF079676
EHEC溶血素 EHEC hemolysin	ehxA	F-CAATAATTGGTGGTGGACCC	583	55	EF088504
EHEC溶血素 EHEC hemolysin	ehxA	R-TTCTTACCTTGGACATCCCG	583	55	EF088504
相变抗原 Phase variation antigen	agn43	F-GACTATGACCGGATTSTGGCAGGCT	499	67	[30]
相变抗原 Phase variation antigen	agn43	R-GTGGCTCCAGCATCARTTGTCAG	499	67	[30]
铁结合蛋白 Iron transport periplasmic-binding protein	sitA	F-AGGGGGCACAACTGATTCTCG	608	57	[31]
铁结合蛋白 Iron transport periplasmic-binding protein	sitA	R-TACCGGGCCGTTTTCTGTGC	608	57	[31]
溶血素 Hemolysin	hlyF	F-GGCGATTTAGGCATTCCGATACTC	599	55	[31]
	hlyF	R-ACGGGGTCGCTAGTTAAGGAG	599	55	[31]
	hlyA	F-AACAAGGATAAGCACTGTTCTGGCT	1177	55	[32]
	hlyA	R-ACCATATAAGCGGTCATTCCCGTCA	1177	55	[32]
耶尔森菌强毒力岛 Yersinia high-pathogenicity island	fyuA	F-TGATTAACCCCGCGACGGGAA	880	55	[32]
耶尔森菌强毒力岛 Yersinia high-pathogenicity island	fyuA	R-CGCAGTAGGCACGATGTTGTA	880	55	[32]
纤维素合成酶基因 Cellulose synthase operon genes	bcsA	F-GTATCGGTAGAAAGCAAACAGG	185	55	[33]
	bcsA	R-GAACGGTACACGAGAAGAGG	185	55	[33]
	bcsB	F-CTCGTGTACCGTTCAGGATTTCT	388	55	[33]
	bcsB	R-CAGCCCAACTTCATTACCCAT	388	55	[33]
Ⅰ型菌毛亚基 Type Ⅰ fimbriae	fimC	F-GGAAATAACATTCTGCTTGC	288	56	[34]
Ⅰ型菌毛亚基 Type Ⅰ fimbriae	fimC	R-TTTGTTGCATCAAGAATACG	288	56	[34]
外膜蛋白基因 Outer membrane protein	ompT	F-ATCTAGCCGAAGAAGGAGGC	559	55	[34]
外膜蛋白基因 Outer membrane protein	ompT	R-CCCGGGTCATAGTGTTCATC	559	55	[34]
铁离子摄取相关基因 Iron uptake-related gene	irp2	F-AAGGATTCGCTGTTACCGGAC	280	55	[34]
铁离子摄取相关基因 Iron uptake-related gene	irp2	R-TCGTCGGGCAGCGTTTCTTCT	280	55	[34]
热敏肠毒素 Heat-stable enterotoxin	LT	F-ATGAGTACTTCGATAGAGG	279	55	[34]
热敏肠毒素 Heat-stable enterotoxin	LT	R-ATGGTATTCCACCTAACGC	279	55	[34]