miR-31-5p对山羊毛囊干细胞增殖和凋亡的影响

冯云奎,王健,马金亮,张柳明,李拥军

Effects of miR-31-5p on the Proliferation and Apoptosis of Hair Follicle Stem Cells in Goat

FENG YunKui,WANG Jian,MA JinLiang,ZHANG LiuMing,LI YongJun

表1 荧光定量PCR引物序列

Table 1 Primer sequence for qPCR

名称 Name 引物名称 Primer name 引物序列（5′-3′） Primer sequence (5′-3′) miR-31-5p Stem-loop RT-miR-31-5p GTCGTATCCAGTGCAGGGTCCGAGGT ATTCGCACTGGATACGACAGCTATGC miR-31-5p-F AGGCAAGATGCTGGCAT miR-31-5p-R GTGCAGGGTCCGAGGT 18S-rRNA ID:493779 18S-F GTGGTGTTGAGGAAAGCAGACA 18S-R TGATCACACGTTCCACCTCATC PCNA ID:102172276 PCNA-F ATCAGCTCAAGTGGCGTGAA PCNA-R TGCCAAGGTGTCCGCATTAT CDK1 ID:10086361 CDK1-F AGATTTTGGCCTTGCCAGAG CDK1-R AGCTGACCCCAGCAATACTT CCND2 ID:102180657 CCND2-F GGGCAAGTTGAAATGGAA CCND2-R TCATCGACGGCGGGTAC Bax ID:100846984 Bax-F TTTCCGACGGCAACTTCAA Bax-R TGAGCACTCCAGCCACAAA Bcl-2 ID:100861254 Bcl-2-F ATGTGTGTGGAGAGCGTCAA Bcl-2-R CCTTCAGAGACAGCCAGGAG MAP3K1 ID:102187530 MAP3K1-F GAGAGTTGGCAGTTGGCAGAG MAP3K1-R CAGTTGTTTGATTCAGTTTGGTTTCC RASA1 ID:102170782 RASA1-F RASA1-R TGCCAGAGGAAGAGTACAGC TTCCATCAAGGTGCTCGCAA GAPDH ID:100860872 GAPDH-F AGGTCGGAGTGAACGGATTC GAPDH-R CCAGCATCACCCCACTTGAT