葡萄Fe-S簇装配基因的鉴定、克隆和表达特征分析
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张璐,宗亚奇,徐维华,韩蕾,孙浈育,陈朝晖,陈松利,张凯,程杰山,唐美玲,张洪霞,宋志忠
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Identification, Cloning, and Expression Characteristics Analysis of Fe-S Cluster Assembly Genes in Grape
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ZHANG Lu,ZONG YaQi,XU WeiHua,HAN Lei,SUN ZhenYu,CHEN ZhaoHui,CHEN SongLi,ZHANG Kai,CHENG JieShan,TANG MeiLing,ZHANG HongXia,SONG ZhiZhong
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表5 Fe-S簇装配基因亚细胞定位预测
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Table 5 Subcellular localization prediction of Fe-S cluster assembly gene
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蛋白位置
Protein location | 蛋白
Protein | 亚细胞定位 Subcellular localization (%) | | 叶绿体Chloroplast | 线粒体Mitochondria | 细胞质Cytosol | 细胞核Nucleus | 内质网膜
Endoplastic reticulum | 液泡膜
Vacular membrane | 细胞质膜
Plasma membrane | 高尔基体
Golgi
| 质体
Plastid | NFS2 | 71.43 | 28.57 | - | - | - | - | - | - | | SUFE1 | 7.14 | - | 21.43 | 57.15 | - | 7.14 | 7.14 | - | | SUFE2 | 92.86 | 7.14 | - | - | - | - | - | - | | SUFE3 | 7.14 | - | 64.29 | 14.29 | 7.14 | - | 7.14 | - | | SUFA | 85.72 | 7.14 | 7.14 | - | - | - | - | - | | NFU1 | 72 | 28 | - | - | - | - | - | - | | NFU2 | 85.72 | - | - | 7.14 | 7.14 | - | - | - | | NFU3 | 57.15 | - | 21.43 | 7.14 | - | - | 14.28 | - | | SUFB | 57.50 | 32.50 | - | 10.00 | - | - | - | - | | SUFC | 85.71 | 14.29 | - | - | - | - | - | - | | SUFD | 68.3 | 21.95 | - | 9.75 | - | - | - | - | | HCF101 | 57.14 | 14.29 | - | - | - | 21.43 | - | 7.14 | | GRXS14 | 92.86 | - | - | - | - | 7.14 | - | - | | GRXS16 | 83.72 | 16.28 | - | - | - | - | - | - | 线粒体
Mitochondria | NFS1 | 35.71 | 35.71 | - | 28.58 | - | - | - | - | | ISD11 | - | 77.42 | - | 16.13 | - | - | 6.45 | - | | ISU1 | 85.72 | - | 14.28 | - | - | - | - | - | | ISA1 | 92.85 | 7.15 | - | - | - | - | - | - | | ISA2 | 78.57 | 7.15 | 14.28 | - | - | - | - | - | | ISA3 | 21.63 | 45.94 | 27.03 | 5.40 | - | - | - | - | | NFU4 | 50 | 14.28 | - | 14.28 | - | 7.14 | - | - | | ADX1 | 14.28 | 78.58 | - | 7.14 | - | - | - | - | | ADXR | 50 | 35.72 | 7.14 | 7.14 | - | - | - | - | | FH | 50 | 35.72 | - | 14.28 | - | - | - | - | | HSCA1 | 14.28 | 85.72 | - | - | - | - | - | - | | HSCA2 | 14.28 | 85.72 | - | - | - | - | - | - | | HSCA3 | 21.42 | - | 78.58 | - | - | - | - | - | | HSCA4 | - | - | - | - | 100 | - | - | - | | HSCA5 | 7.14 | 7.14 | 78.58 | - | - | - | 7.14 | - | | HSCA6 | - | - | - | - | 100 | - | - | - | | HSCA7 | 43.76 | - | 15.62 | 15.62 | - | 12.5 | - | 12.5 | | HSCB | 71.44 | 14.28 | - | 14.28 | - | - | - | - | | INDL | 14.28 | - | 78.58 | 7.14 | - | - | - | - | | IBA57 | 64.30 | 14.28 | 7.14 | 7.14 | - | - | 7.14 | - | | GRXS15 | 42.86 | 57.14 | - | - | - | - | - | - | 细胞质
Cytosol | ATM3 | 7.14 | 7.14 | - | - | 28.57 | - | 57.15 | - | | ERV1 | 14.28 | - | 14.28 | 57.16 | - | 7.14 | 7.14 | - | | NAR1 | 21.42 | 7.14 | 42.88 | 21.42 | - | 7.14 | - | - | | NBP35-1 | 57.16 | - | 21.42 | 21.42 | - | - | - | - | | NBP35-2 | 14.28 | - | 78.58 | 7.14 | - | - | - | - | | TAH18 | 7.14 | - | 35.71 | 57.15 | - | - | - | - | | DRE2 | 21.42 | - | 21.42 | 57.16 | - | - | - | 7.14 | | CIA1 | 14.28 | - | 28.58 | 53 | - | 7.14 | - | - | | CIA2 | 7.14 | - | 64.30 | 21.42 | - | - | - | 7.14 | | CIA3 | - | - | 100 | - | - | - | - | - | | MMS19 | 14.28 | - | 28.57 | 35.73 | - | 14.28 | - | 7.14 |
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