葡萄Fe-S簇装配基因的鉴定、克隆和表达特征分析

张璐,宗亚奇,徐维华,韩蕾,孙浈育,陈朝晖,陈松利,张凯,程杰山,唐美玲,张洪霞,宋志忠

Identification, Cloning, and Expression Characteristics Analysis of Fe-S Cluster Assembly Genes in Grape

ZHANG Lu,ZONG YaQi,XU WeiHua,HAN Lei,SUN ZhenYu,CHEN ZhaoHui,CHEN SongLi,ZHANG Kai,CHENG JieShan,TANG MeiLing,ZHANG HongXia,SONG ZhiZhong

表2 本研究所用特异性表达引物

Table 2 Specific expression primers used in this study

基因 Gene 上游引物 Forward primer (5′-3′) 下游引物 Reverse primer (5′-3′) 产物长度 Product length (bp) ADX1 AACCACAATCGGGGACCAAG GTGGAACAGGCAAGTGAACC 250 ADXR TGGGCATCCAGACTGCAAAA TGGTCCACGTCTTCCTACCA 211 ATM3 CACCGACACCCTAGTTGGAC TAGCCCCAACCAAGAAACCC 232 CIA1 GCAACGTTCTCCCTCCACTT GACCCGGATGCATTCCAAGA 231 CIA2 TGTTTTTCCCTGAGGTGGACAT GCCAATTACTGTTGCCATGCT 164 CIA3 AAACTTCTGCGCAGTTTGCC GGAGCCAGACATTCATCAACC 167 DRE2 ATAGCACATTGGCCCTCACA TGTCCCACCAGGCTTCAAAA 248 ERV1 TCGGCAGCAGAAGAAGGATG CCCCACCTTGCATCAACTCT 222 FH GGCCGCCATTGATTACAGTTC GCTCACAGGAGAGGACAACC 235 GRXS14 GGAACCAAGGAGTTCCCACA CGCCTTCTCCAACTGTTCCT 243 GRXS15 TGAAAGGGGTGCCTGATGTG TTGTGGAAATGTGGGCCAGT 158 GRXS16 AAACTGAGCCGATTCCCCTG CTCTGGCACCGATTTCCTGT 167 HCF101 GAGTGGGGAGAGCTGGACTA AACAACAGCAACACAGGGGA 192 HSCA1 CTCGCTTCCCCTTCTCTCTC CCACTGATGGTGTAGTCCGAG 235 HSCA2 CCATTCAGCTCAAAGCCTGC GGGAGCTCTGAGTTTAGTTCCA 243 HSCA3 AGAGAGGTTTGCAAAGGAGGA GTCCCTTTCCTGATGACTTTCC 244 HSCA4 ACCAATGACAAGGAGGAAGACA CACTCGAGGGCCTCTTTCAC 176 HSCA5 CAGAGACTGCCGAGAAAGCA GCGTTGAACCACCAACAAGG 246 HSCA6 GTCAAGGCTGAGGACAAGGG GACAGCTTCCACCTCATCAATC 165 HSCA7 TACACGAGTTTGCAGAAGAGG AGCCCCACCACTCCTCATAAA 177 HSCB TCCATGTTCCTCAACTGCCC ACAAGAGTCGCAAGCCAGAA 181 IBA57 GTGGATGCCACTGTCTTGGA ATGTGCCAGCAGGATCAACA 202 INDL AGTGGTTCTGCGGGGTTTAG ATCAGCATCAAGCACACCCA 168 ISA1 CCTAACCCTAACGGACGCTG CCAATGACATGCATGAGAGCC 213 ISA2 GCCCTCCACTTCGGTTTCTC CCAGAGCATCCACCCTGTTT 219 ISA3 TTGAGTGTCGAGACTGGTGG TGCTCACCAGGAAAGCAGAA 193 ISD11 ACGCGATTTCTCGGACTACAAT TATTCCAACTGCCTTCTGGC 156 ISU1 CCATCGCCTCTTCCTCTGTT AGTCTTTGCTCGCTTGGCT 188 MMS19 AGCTTGCTGTTGGGAGTTGT GGGTTTGAGGACGAACTGCT 231 NAR1 AAAGCTGAGGTTGTGACGGG AAAGGAAGCCCTTGACTGGG 204 NBP35-1 TTCCTAGTGGTTGATGCCCC ACACCTCACTGAAATCTGTCAC 232 NBP35-2 AGTGGTTCTGCGGGGTTTAG ATCAGCATCAAGCACACCCA 168 NFS1 GACAAATCAAAAAGCCATTTTCCAA CACGTACTGCTCTCGGGTT 150 NFS2 GTTCAGGTGGGGTTCCGTAG AGGCTTCTGTGAGGTTGCTG 183 NFU1 CAAAACTCAGCGTTTCGCCT ATTTGTGGGCCGAGTAGAGC 163 NFU2 CCTACTGCTGCAGAGCCAAA GCAACAGCCTTGACAACTCG 172 NFU3 AAACACGCCCTAGAGCCTTC AGCCTTGACAACTCGGCTAC 150 NFU4 GGGAAGCCACTGTTCCTTGA CGATACTCGATGTCCCCACC 152 SUFA GCCCTCCACTTCGGTTTCTC CCAGAGCATCCACCCTGTTT 219 SUFB ACAAAGTTTGCAGATGACAGGA CTAGCCCCACTGCATAGACC 221 SUFC CCATGCAGTCATGGGGAAGA ACCTGGGATCTCTACTGGGG 190 SUFD AATTGCCCACTGGCGTTTTC ATATCCCCACCCTCAACGGA 217 SUFE1 TGGACATGCAGGTGTTAGGG TTCCCAAGTTGATTTCCCTGTCT 219 SUFE2 TGATTCGGGTTTTGGACGGA AGATATTGCAGTGCCAGAGACA 227 SUFE3 TCCTGAGGTCCAAGGTGTCT GCATCTGCCAAAGAGCAACC 243 TAH18 GCGCTATTTCTTTGAGGCAAG AATGGCATTTGCACTGAAGGG 174