葡萄Fe-S簇装配基因的鉴定、克隆和表达特征分析

张璐,宗亚奇,徐维华,韩蕾,孙浈育,陈朝晖,陈松利,张凯,程杰山,唐美玲,张洪霞,宋志忠

Identification, Cloning, and Expression Characteristics Analysis of Fe-S Cluster Assembly Genes in Grape

ZHANG Lu,ZONG YaQi,XU WeiHua,HAN Lei,SUN ZhenYu,CHEN ZhaoHui,CHEN SongLi,ZHANG Kai,CHENG JieShan,TANG MeiLing,ZHANG HongXia,SONG ZhiZhong

表1 葡萄Fe-S簇装配基因CDS扩增所需引物

Table 1 Primers used for CDS amplification of grape Fe-S cluster assembly genes

基因Gene 上游引物 Forward primer (5′-3′) 下游引物 Reverse primer (5′-3′) 产物长度 Product length (bp) ADX1 ATGTTCGCTTCTAGACTTTCA TTAGTGTGGTTTTGGAATAAA 597 ADXR ATGGCGTTTTGTCGTGCGAAG TCATTCCATGGTAACTTTCAG 1461 ATM3 ATGGCGGGCGTTGCTTCTTGC TCACGCCTCCAATTTAATGGT 2181 CIA1 ATGGATTTCTCGGAAGAGGGC TCAGGTTATAGAAGCCAGCTC 1035 CIA2 ATGTTTTTCCCTGAGGTGGAC TTAGAGTTCATTGGAGTAGAG 360 CIA3 ATGTTCTTAAATCATATTAGA TCAACCATATGATGGAGCCAG 363 DRE2 ATGTTGCAAAATAGCACATTG TTAAATGTCAGCCACAAGAA 819 ERV1 ATGTCTGAAACCCCATTTCAT TTAATGTGATCTCTCACCAAA 597 FH ATGGCTTCTGCTTCTTCTTCC TTAGGAAAGGCTGATGGGTGT 594 GRXS14 ATGCTTATGCTTATATCAATA TCAGGAGCACATCGCCTTCTC 552 GRXS15 ATGGCAAGGTCACTATCTAAC TCATTCGGACTTTTCCTGGGG 513 GRXS16 ATGGCAACAATCAGTCTATCT CTACTTCTTAAATAAACCAAC 894 HCF101 ATGAGGCTAACAAACACTCTT TCATGCCTGTGCAGGAGTTAG 1671 HSCA1 ATGGCTGCCTCCGTTTTGCTC CTACTTCTTCACCTCTTCATA 1842 HSCA2 ATGGCCACTTCGGTGCTTCTT CTACTTCTTGGCCTGCTCATA 1716 HSCA3 ATGTCGGAGTGCCCAGCGATC TCACATCACCATCCGGTCCCTT 1503 HSCA4 ATGTACGGTGCGTGGGGTCTA CTACAGCTCATCATGGGAGTC 1947 HSCA5 ATGGACGAGGTGGGTGAGGA TCACTTGCTCTCGGTGAAGTC 1677 HSCA6 ATGGAAAGCTCGTGGAGAAGA TCAAAGTTCATCATGGGAATC 1821 HSCA7 ATGGCGGTGAAGAACAGGGCG TCAGAACTGGAAGAAGCCCCA 1731 HSCB ATGTGGAAGAAGAATCTATGG TCAGAGTTTCTTCATGATTTC 804 IBA57 ATGGCCTCGCTTCTCAAGTCC CTAAGCGGACGCAGTATCTTG 1113 INDL ATGGAGTCACCGACCCAGGGA TCACAGTGAAATCTCTGGCCG 1026 ISA1 ATGGCATCTTCATTTCTCGCG CTACTTAGCAGCTCCAGGATT 402 ISA2 ATGCCCCAGCCCTCCACTTCG TCACATTTCAGCAGCAAAGGA 453 ISA3 ATGTCAAGATCGGTGCTTCGG CTATTCTTTCACCATGAAAGA 465 ISD11 ATGGCATCAACACCTTCGAAA TTATTCCAACTGCCTTCTGGC 219 ISU1 ATGAAGCTTCAAATCAAGGTC CTACTCAGTCTTTGCTCGCTT 273 MMS19 ATGGCACAACTGAGTCAATTG TCAGAAATGAAGACTTCTGGA 3441 NAR1 ATGTCGGAGAAGTTCTCGGCA TCACCAGTTGTGTAATTGAGA 1437 NBP35-1 ATGGAGAACGGCGACAGCAAT TCAAGTGGTAGACAGTGCCTG 957 NBP35-2 ATGGAGTCACCGACCCAGGGA TCACAGTGAAATCTCTGGCCG 1026 NFS1 ATGGCTTCCAGGCTTCATTCT TTAGGTTATTTGTTTTTGTAG 3267 NFS2 ATGGCAAGTGTTCTGAAATT CTACTTGAAAGAAGAGAAGA 1392 NFU1 ATGGCGTCTCTCACAGCAACA CTAGTCACTGAAGACAACATT 642 NFU2 ATGCAAGGCGTGGTGGTGGCG CTATAGAAGCTGAACTGCTGC 684 NFU3 ATGGGCTTCACAGCATTCACA TTATCCATCTATCAACTGGAC 714 NFU4 ATGTTCATCCAAACTCAATCC TTAATCCATCTGGCCAGATAG 609 SUFA ATGCCCCAGCCCTCCACTTCG TCACATTTCAGCAGCAAAGGA 453 SUFB ATGGCTTCTCTACTGGCCAAC CTAACCGACTGATCCTTCAAGC 1536 SUFC ATGGCTCGATTCTGCTGCGCC TTACAGTTGGGCCTCAGAAAT 966 SUFD ATGGCTGCTACATTGCTTTCA TCATGAGGAACCTTTAAGTGT 1443 SUFE1 ATGTCTCTGTCCAATCTCCAA TCAACATAGATTCCCAAGTTG 1224 SUFE2 ATGGACTCTGCAACTTTGGG TCATATCCTGAAAGGACTCTG 855 SUFE3 ATGGGGTGTACGGCGCAGGTG TCAACTTATTGAAGTTGATC 1806 TAH18 ATGAATGGGAGGGAGAAGCAG TCAAGACCATGCTTCCACATG 1899