异色瓢虫丙酮酸激酶基因序列分析及其调控海藻糖代谢功能

葛欣竺,史宇星,王莎莎,刘智慧,蔡文杰,周敏,王世贵,唐斌

Sequence Analysis of Harmonia axyridis Pyruvate Kinase Gene and Its Regulation of Trehalose Metabolism

GE XinZhu,SHI YuXing,WANG ShaSha,LIU ZhiHui,CAI WenJie,ZHOU Min,WANG ShiGui,TANG Bin

图4 基于氨基酸序列构建的HaPYK与其他昆虫丙酮酸激酶的系统进化树 图中分支上的数字表示Bootstrap验证中基于1000次重复该节点的可信度,丙酮酸激酶名称后面括号里的字母及数字为GenBank登录号The number on the branch in the figure represents the reliability of the node based on 1000 repetitions in Bootstrap verification, the letters and numbers in parentheses following the name of pyruvate kinase are the GenBank accession numbers。HsPYK：跳镰猛蚁丙酮酸激酶Harpegnathos saltator pyruvate kinase;ObPYK：毕氏卵角蚁丙酮酸激酶Ooceraea biroi pyruvate kinase;BgPYK：德国小蠊丙酮酸激酶Blattella germanica pyruvate kinase;MsPYK：东方黏虫丙酮酸激酶Mythimna separata pyruvate kinase;AaPYK X1：埃及伊蚊丙酮酸激酶亚型X1 Aedes aegypti pyruvate kinase isoform X1;LdPYK1、LdPYK2：马铃薯甲虫丙酮酸激酶1、丙酮酸激酶2 Leptinotarsa decemlineata pyruvate kinase 1, pyruvate kinase 2;HaPYK1-1、HaPYK1-2、HaPYK2：异色瓢虫丙酮酸激酶1-1、丙酮酸激酶1-2、丙酮酸激酶2 Harmonia axyridis pyruvate kinase 1-1, pyruvate kinase 1-2, and pyruvate kinase 2;PpPYK-like：萤火虫丙酮酸激酶样Photinus pyralis pyruvate kinase-like;TcPYK：赤拟谷盗丙酮酸激酶Tribolium castaneum pyruvate kinase;DpPYK-like：中欧山松大小蠢丙酮酸激酶样Dendroctonus ponderosae pyruvate kinase-like;AgPYK-like：光肩星天牛丙酮酸激酶样Anoplophora glabripennis pyruvate kinase-like;NlPYK X1：褐飞虱丙酮酸激酶亚型X1 Nilaparvata lugens pyruvate kinase isoform X1

Fig. 4 Phylogenetic tree of HaPYK and pyruvate kinases from other insect species based on amino acid sequence