异色瓢虫丙酮酸激酶基因序列分析及其调控海藻糖代谢功能

葛欣竺,史宇星,王莎莎,刘智慧,蔡文杰,周敏,王世贵,唐斌

Sequence Analysis of Harmonia axyridis Pyruvate Kinase Gene and Its Regulation of Trehalose Metabolism

GE XinZhu,SHI YuXing,WANG ShaSha,LIU ZhiHui,CAI WenJie,ZHOU Min,WANG ShiGui,TANG Bin

表1 dsRNA合成和实时荧光定量PCR所需引物

Table 1 Primers used for dsRNA synthesis and real-time fluorescent quantitative PCR (qRT-PCR)

引物名称 Primer name 正向引物 Forward primer (5′-3′) 反向引物 Reverse primer (5′-3′) 用途 Application dsHaPYK1-1 ACATGGTCTTCGCCTCCTTCA TGCAGCCAGACACTTAGCAGA 合成dsRNA dsRNA synthesis dsHaPYK1-2 ACAAGGGACGCTGACACTGT TCAAGTGTGCAGATCGTCCAGT dsHaPYK2 ATAGTAACGTCGCGGTCACT GATCCTCCTTTGATGCGCTG dsGFP AAGGGCGAGGAGCTGTTCACCG CAGCAGGACCATGTGATCGCGC dsHaPYK1-1-T7 T7-ACATGGTCTTCGCCTCCTTCA T7-TGCAGCCAGACACTTAGCAGA dsHaPYK1-2-T7 T7-ACAAGGGACGCTGACACTGT T7-TCAAGTGTGCAGATCGTCCAGT dsHaPYK2-T7 T7-ATAGTAACGTCGCGGTCACT T7-ATAGTAACGTCGCGGTCACT dsGFP-T7 T7-AAGGGCGAGGAGCTGTTCACCG T7-CAGCAGGACCATGTGATCGCGC qHaPYK1-1 AGTTGACCACAGACAAGGCT AGACAGCAGGTAGATCGACG qRT-PCR qHaPYK1-2 CTGGACGATCTGCACACTTG CACGAGCGTTCACATCTTGT qHaPYK2 GCAAACTTGTTCAGCCAGGA GCAGCCTGATGTTTCTCTCG qHaTRE1-1 CTTCGCCAGTCAAATCGTCA CCGTTTGGGACATTCCAGAT qHaTRE1-2 TGACAACTTCCAACCTGGTAATG TTCCTTCGAGACATCTGGCTTA qHaTRE1-3 ACAGTCCCTCAGAATCTATCGTC GGAGCCAAGTCTCAAGCTCATC qHaTRE1-4 TTACTGCCAGTTTGATGACCAT CATTTCGCTAATCAGAAGACCCT qHaTRE1-5 TGATGATGAGGTACGACGAGA GTAGCAAGGACCTAACAAACTG qHaTRE2-like TTCCAGGTGGGAGATTCAGG GGGATCAATGTAGGAGGCTGTG qHaTRE2 CAATCAGGGTGCTGTAATGTCG CGTAGTTGGCTCATTCGTTTCC qHaTPS GACCCTGACGAAGCCATACC AAAGTTCCATTACACGCAC qrp49 GCGATCGCTATGGAAAACTC TACGATTTTGCATCAACAGT