绿僵菌mad2敲除株构建及其生物学和诱导植物响应的功能分析

蔡霓,闫多子,农向群,王广君,涂雄兵,张泽华

Adhesin Gene mad2 Knockout and Functional Effects on Biological Characteristics and Inducing Plant Responses in Metarhizium anisopliae

CAI Ni,YAN DuoZi,NONG XiangQun,WANG GuangJun,TU XiongBing,ZHANG ZeHua

表2 候选基因及其特异性引物

Table 2 Candidate genes and their specific primers

基因Gene 引物序列Primer sequence 基因Gene 引物序列Primer sequence RPL7 5′-ACAGTTGGTCCTCACTTCAG-3′ LTP1 5′-CAACCAACCAATCACCCTC-3′ 5′-GCTCATTTATGTAAGCTTCCCT-3′ 5′-GGGCTAATCTTGTATGGGATG-3′ CCaMK 5′-ATCCGCCTTTCATTGCTC-3′ NRT24 5′-CCCACGGAGAAGAGAATCATC-3′ 5′-GAACATCTTTGGCTACATCACC-3′ 5′-GGAATGGCAAACATCATAGCAC-3′ ABCC2 5′-CTCATAGGCATTGTCAGCAC-3′ DELLA 5′-CCTCCACCATCACAACTTCC-3′ 5′-GTGAATCGGATGTTGTTGTC-3′ 5′-CATTCTCCTGCGAGTCAACC-3′ SYMRK 5′-AAGCAATGTGGAGAGTGGTG-3′ CaM 5′-TGATGGCTAAGTGAGGACC-3′ 5′-AGATGAAGTAGAGGGCGGAA-3′ 5′-AATGAAAGCAAGGGACAGAG-3′