绿僵菌mad2敲除株构建及其生物学和诱导植物响应的功能分析

蔡霓,闫多子,农向群,王广君,涂雄兵,张泽华

Adhesin Gene mad2 Knockout and Functional Effects on Biological Characteristics and Inducing Plant Responses in Metarhizium anisopliae

CAI Ni,YAN DuoZi,NONG XiangQun,WANG GuangJun,TU XiongBing,ZHANG ZeHua

表1 载体构建及目标片段特异性引物

Table 1 Specific primer pairs for vector construction and target fragment

引物名称Primer name 序列Sequence 片段长度Sequence length (bp) Hyg-F 5′-ATGCCTCAGCGAATTCGATAACTG-3′ 1426 Hyg-R 5′-GTTCCCGGTCGGCATCTACTCTA-3′ mad2 S1-F 5′-CCCATTGTCTC CACGCACACT-3′ 1046 mad2 S1-R 5′-GTTATCGAATTCGCTGAGGCATCGAAGGTATAAGGTGAGAGCGGTTG-3′ mad2 S2-F 5′-TAGAGTAGATGCCGACCGGGAACGTTGACCGCACAGCATAT ATCCTGG-3′ 1174 mad2 S2-R 5′-CCCAGCAACAAGCCCACATGCAGCAA-3′ Hyg S2-F 5′-CAGCTTCGATGTAGGAGGGCGTGG-3′ 1174 + 936 Hyg S1-R 5′-GGCAGTCCTCGGCCCAAAGCATCAG-3′ 1046 + 945 OUT-mad2-F 5′-ATGAAGTCTTTCATTGCTGTTGCGG-3′ 921 OUT-mad2-R 5′-CTAGATCAGGAGAGCGGCGATGAGG-3′