我国苹果斑点落叶病菌携带dsRNA分析及一种dsRNA病毒的鉴定

曹钰晗,李紫腾,张静怡,张静娜,胡同乐,王树桐,王亚南,曹克强

Analysis of dsRNA Carried by Alternaria alternata f. sp. mali in China and Identification of a dsRNA Virus

CAO YuHan,LI ZiTeng,ZHANG JingYi,ZHANG JingNa,HU TongLe,WANG ShuTong,WANG YaNan,CAO KeQiang

表2 本试验所用引物

Table 2 Primers used in this study

引物名称 Primer name 引物序列 Primer sequence (5′-3′) 条带大小 Product size (bp) 用途 Purpose 来源 Source CRV1-5F GCAAAAAAGAACTAAAGGAC 800 AaCV2 ORF5的扩增 Amplification of AaCV2 ORF5 自行设计 Designed in this study CRV1-5R ACACACAAATGGGATACC CRV1-1F ATCTCAAGGTACCGGAGTCA 450 AaCV2 RT-PCR验证 Identification of AaCV2 by RT-PCR 自行设计 Designed in this study CRV1-1R GCTGCTTAAACATCACCTGCA