转基因玉米NK603转化体/zSSIIb内标基因二重微滴数字PCR方法的建立及应用

肖芳,李俊,王颢潜,翟杉杉,陈子言,高鸿飞,李允静,吴刚,张秀杰,武玉花

Establishment and Application of A Duplex ddPCR Method to Quantify the NK603/zSSIIb Copy Number Ratio in Transgenic Maize NK603

XIAO Fang,LI Jun,WANG HaoQian,ZHAI ShanShan,CHEN ZiYan,GAO HongFei,LI YunJing,WU Gang,ZHANG XiuJie,WU YuHua

图2 NK603转化体与不同内标基因的二重ddPCR退火温度优化热图 A：NK603/zein-ENDO二重ddPCR的NK603转化体和zein-ENDO一维热图;B：NK603/Adh1双重ddPCR的NK603转化体和Adh1一维热图;C：NK603/hmg双重ddPCR的NK603转化体和hmg一维热图;D：NK603/zSSIIb二重ddPCR的NK603转化体和zSSIIb一维热图;E：NK603/zein-taq二重ddPCR的NK603转化体和zein-taq一维热图;F：60℃退火温度下NK603/zSSIIb二重ddPCR的二维热图

Fig. 2 Hot plots of duplex ddPCR of NK603 event and different reference genes A: One-dimension (1-D) hot plots of NK603 event and zein-ENDO gene for NK603/zein-ENDO duplex ddPCR; B: 1-D hot plots of NK603 event and Adh1 gene for NK603/Adh1 duplex ddPCR; C: 1-D hot plots of NK603 event and hmg gene for NK603/hmg duplex ddPCR; D: 1-D hot plots of NK603 event and zSSIIb gene for NK603/zSSIIb duplex ddPCR; E: 1-D hot plots of NK603 event and zein-taq gene for NK603/zein-taq duplex ddPCR; F: Two-dimension (2-D) hot plot of NK603/zSSIIb duplex ddPCR at annealing temperature of 60℃