转基因玉米NK603转化体/zSSIIb内标基因二重微滴数字PCR方法的建立及应用

肖芳,李俊,王颢潜,翟杉杉,陈子言,高鸿飞,李允静,吴刚,张秀杰,武玉花

Establishment and Application of A Duplex ddPCR Method to Quantify the NK603/zSSIIb Copy Number Ratio in Transgenic Maize NK603

XIAO Fang,LI Jun,WANG HaoQian,ZHAI ShanShan,CHEN ZiYan,GAO HongFei,LI YunJing,WU Gang,ZHANG XiuJie,WU YuHua

图1 融合序列及标准质粒分子pUC57-NK603结构示意图 A：NK603转化体特异性序列与玉米内标基因融合序列。单下划线序列为NK603转化体特异性序列,阴影所示序列为间隔序列,无标记序列为Adh1序列,下划虚线序列为zSSIIb序列,双下划线序列为hmg序列,方框所示序列为zein序列。B：标准质粒分子pUC57-NK603结构示意图

Fig. 1 Schematic diagram of the fusion fragment and standard plasmid molecule pUC57-NK603 A: The fusion fragment of NK603 event-specific sequence and maize reference gene sequences. The NK603 event-specific sequence was marked with single underline, the spacer sequence was shaded, the Adh1 sequence was shown without marks, the zSSIIb sequence was marked with underlined dashed line, the hmg sequence was marked with double underline, and the zein sequence was shown in the box. B: Schematic diagram of the standard plasmid molecule pUC57-NK603