转基因玉米NK603转化体/zSSIIb内标基因二重微滴数字PCR方法的建立及应用

肖芳,李俊,王颢潜,翟杉杉,陈子言,高鸿飞,李允静,吴刚,张秀杰,武玉花

Establishment and Application of A Duplex ddPCR Method to Quantify the NK603/zSSIIb Copy Number Ratio in Transgenic Maize NK603

XIAO Fang,LI Jun,WANG HaoQian,ZHAI ShanShan,CHEN ZiYan,GAO HongFei,LI YunJing,WU Gang,ZHANG XiuJie,WU YuHua

表1 PCR引物/探针信息

Table 1 Primer and probe information for PCR assays

靶标 Target 引物/探针名称 Primer/probe name 序列 Sequence (5'-3') 片段长度 Fragment size (bp) 参考文献 Reference NK603转化体 NK603 event 603-QF ATGAATGACCTCGAGTAAGCTTGTTAA 108 [30] 603-QR AAGAGATAACAGGATCCACTCAAACACT 603-QP TGGTACCACGCGACACACTTCCACTC zein-ENDO zein-ENDO F GCATTGTTCGCTCTCCTAGC 110 [31] zein-ENDO R TACTGCATCCATGGGTTCAT zein-ENDO P TTCCAGGGCACTTGCCACCA zein-taq zein-taqze F GCCATTGGGTACCATGAACC 104 [31] zein-taqze R TACTGCATCCATGGGTTCAT zein-taqze P AGCTTGATGGCGTGTCCGTCCCT hmg hmg F TTGGACTAGAAATCTCGTGCTGA 79 [31] hmg R GCTACATAGGGAGCCTTGTCCT hmg P CAATCCACACAAACGCACGCGTA zSSIIb zSSIIb F CGGTGGATGCTAAGGCTGATG 88 [32] zSSIIb R AAAGGGCCAGGTTCATTATCCTC zSSIIb P TAAGGAGCACTCGCCGCCGCATCTG Adh1 Adh1 F CGTCGTTTCCCATCTCTTCCTCC 135 [31] Adh1 R CCACTCCGAGACCCTCAGTC Adh1 P AATCAGGGCTCATTTTCTCGCTCCTCA