小麦TaNAC基因基于可变剪切和microRNA的转录后调控分析

吕士凯,马小龙,张敏,邓平川,陈春环,张宏,刘新伦,吉万全

Post-transcriptional Regulation of TaNAC Genes by Alternative Splicing and MicroRNA in Common Wheat (Triticum aestivum L.)

LÜ ShiKai,MA XiaoLong,ZHANG Min,DENG PingChuan,CHEN ChunHuan,ZHANG Hong,LIU XinLun,JI WanQuan

图6 部分TaNAC基因的结构变异转录本转录调控活性的比较分析 a：连接不同目的片段的重组pGBKT7载体转化至酵母菌株Y2H Gold的阳性菌株在SD/-Trp/X-α-Gal固体培养基的生长情况;b：TaNAC008_3A的2个结构变异转录本及阴阳性对照重组载体对应的酵母菌株在SD/-Trp、SD/-Trp/-His/-Ade和SD/-Trp/AbA固体培养基的生长情况;a和b中的A—E为样本的行代号,1—4为样本的列代号,行列代号组合后的“A1—E1”代表转化有连接着不同目的片段的重组pGBKT7载体的Y2H Gold阳性酵母菌株,具体见表5;E2：转化了pGBKT7-53+pGADT7-T载体的阳性对照组酵母菌株,E3：转化有pGBKT7空载体的阴性对照组酵母菌株

Fig. 6 Comparative analysis of transcriptional activation of some structural variant transcripts of TaNAC genes a: Growth of yeast Y2H Gold positive strain transformed by recombinant pGBKT7 vector with different target fragments in SD/Trp/X-α-gal solid medium. b: Growth of yeast strains corresponding to the recombinant vectors of the two structural variant transcripts of TaNAC008_3A and the negative and positive control vectors in SD/-Trp, SD/-Trp/-his/-Ade and SD/-Trp/ABA solid media. In a and b, A-E are the row numbers of the samples, and 1-4 are the column numbers. After combination of the row and column numbers, A1-E1 represent Y2H Gold positive yeast strains transformed with recombinant pGBKT7 vectors with different target fragments, as detailed in Table 5. E2: The positive control yeast strain transformed with pGBKT7-53+pGADT7-T vectors, E3: The negative control strain transformed with pGBKT7 vector