小麦TaNAC基因基于可变剪切和microRNA的转录后调控分析

吕士凯,马小龙,张敏,邓平川,陈春环,张宏,刘新伦,吉万全

Post-transcriptional Regulation of TaNAC Genes by Alternative Splicing and MicroRNA in Common Wheat (Triticum aestivum L.)

LÜ ShiKai,MA XiaoLong,ZHANG Min,DENG PingChuan,CHEN ChunHuan,ZHANG Hong,LIU XinLun,JI WanQuan

表6 小麦参考基因组（IWGSC RefSeq v1.1）注释的TaNAC转录本与小麦tae-miRNA的靶向关系预测结果

Table 6 The predicted relationship of the TaNAC transcripts in IWGSC RefSeq v1.1 and the tae-miRNA

miRNA名称 miRNA ID 期望值 Expectation 抑制方式 Inhibition TaNAC转录本名称 TaNAC transcripts ID 靶基因 长度 Length (bp) 是否可 变剪切 AS or not NAM结构域编码起止位置 NAM coding start_end (bp) miRNA结合起止位置 Target miRNA start_end (bp) 靶基因与miRNA匹配的序列 Target aligned fragment (5′-3′) 匹配方式 Alignment tae-miR164 1 裂解 Cleavage TraesCS5A02G049100.1 918 是 Yes 40—423 653—673 AGCAAGUGCCCUGCUUCUCCA ::: :::::::::::::::: TraesCS5A02G049100.2 921 是 Yes 40—423 656—676 AGCAAGUGCCCUGCUUCUCCA ::: :::::::::::::::: TraesCS5B02G054200.1 921 是 Yes 40—423 656—676 AGCAAGUGCCCUGCUUCUCCA ::: :::::::::::::::: TraesCS5B02G054200.2 924 是 Yes 40—423 659—679 AGCAAGUGCCCUGCUUCUCCA ::: :::::::::::::::: TraesCS5D02G059700.1 909 是 Yes 40—423 644—664 AGCAAGUGCCCUGCUUCUCCA ::: :::::::::::::::: TraesCS5D02G059700.2 912 是 Yes 40—423 647—667 AGCAAGUGCCCUGCUUCUCCA ::: :::::::::::::::: TraesCS7A02G334800.1 1092 否 No 64—444 770—790 AGCUCGUGCCCUGCUUCUCCA :: ::::::::::::::::: TraesCS7A02G464100.1 924 否 No 34—435 653—673 AGCAAGUGCCCUGCUUCUCCA ::: :::::::::::::::: TraesCS7A02G464800.1 927 否 No 34—438 656—676 AGCAAGUGCCCUGCUUCUCCA ::: :::::::::::::::: TraesCS7B02G246300.1 1089 否 No 55—444 767—787 AGCUCGUGCCCUGCUUCUCCA :: ::::::::::::::::: TraesCS7B02G364600.1 924 否 No 34—435 653—673 AGCAAGUGCCCUGCUUCUCCA ::: :::::::::::::::: TraesCS7B02G365300.1 918 否 No 34—429 647—667 AGCAAGUGCCCUGCUUCUCCA ::: :::::::::::::::: TraesCS7D02G342300.1 1089 否 No 64—444 767—787 AGCUCGUGCCCUGCUUCUCCA :: ::::::::::::::::: TraesCS7D02G451700.1 939 否 No 34—438 656—676 AGCAAGUGCCCUGCUUCUCCA ::: :::::::::::::::: TraesCS7D02G452500.1 927 否 No 34—435 656—676 AGCAAGUGCCCUGCUUCUCCA ::: :::::::::::::::: 1.5 裂解 Cleavage TraesCS2A02G338300.1 1068 否 No 40—414 680—700 CGCACGUGACCUGCUUCUCCA ::::::: :::::::::::: TraesCS2B02G343600.1 1065 否 No 40—414 680—700 CGCACGUGACCUGCUUCUCCA ::::::: :::::::::::: TraesCS2D02G324700.1 1062 否 No 40—414 677—697 CGCACGUGACCUGCUUCUCCA ::::::: :::::::::::: 2 裂解 Cleavage TraesCS7A02G305200.1 870 否 No 34—414 665—685 AGCAAGUGUCCUGCUUCUCCG ::: :::.:::::::::::. TraesCS7B02G205600.1 879 否 No 34—414 674—694 AGCAAGUGUCCUGCUUCUCCG ::: :::.:::::::::::. TraesCS7D02G302000.1 876 否 No 34—420 671—691 AGCAAGUGUCCUGCUUCUCCG ::: :::.:::::::::::. miRNA名称 miRNA ID 期望值 Expectation 抑制方式 Inhibition TaNAC转录本名称 TaNAC transcripts ID 靶基因 长度 Length (bp) 是否可 变剪切 AS or not NAM结构域编码起止位置 NAM coding start_end (bp) miRNA结合起止位置 Target miRNA start_end (bp) 靶基因与miRNA匹配的序列 Target aligned fragment (5′-3′) 匹配方式 Alignment tae-miR1128 3.5 裂解 Cleavage TraesCS3A02G339600.1 945 是 Yes 97—480 246—266 GUUCGGGAGCAGGGAGUGGUA :::::. : :::::::.::: TraesCS3A02G339600.2 882 是 Yes 97—651 371—391 GUUCGGGAGCAGGGAGUGGUA :::::. : :::::::.::: TraesCS3B02G371200.1 939 否 No 85—468 234—254 GUUCGGGAGCAGGGAGUGGUA :::::. : :::::::.::: TraesCS3D02G333100.1 951 否 No 97—480 246—266 GUUCGGGAGCAGGGAGUGGUA :::::. : :::::::.::: TraesCS4B02G328800.1 1044 否 No 34—414 180—200 GAUCGGGGAGAGGGAGUGGUA ::::. ::::::::.::: TraesCS4B02G328900.1 1059 否 No 49—429 195—215 GAUCGGGGAGAGGGAGUGGUA ::::. ::::::::.::: TraesCS4B02G329100.1 1059 否 No 52—432 198—218 GAUCGGGGAGAGGGAGUGGUA ::::. ::::::::.::: TraesCS4D02G325800.1 1065 否 No 52—432 198—218 GAUCGGGGAGAGGGAGUGGUA ::::. ::::::::.::: TraesCS5A02G500400.1 1017 否 No 22—402 195—215 GAUCGGGGAGAGGGAGUGGUA ::::. ::::::::.::: TraesCS5A02G500500.1 1110 否 No 52—432 168—188 AAUCGGGGAGAGGGAGUGGUA ::::. ::::::::.::: TraesCS5A02G500600.1 1071 否 No 52—432 198—218 GAUCGGGGAGAGGGAGUGGUA ::::. ::::::::.::: TraesCS5A02G500700.1 2085 否 No 19—399 198—218 GAUCGGGGAGAGGGAGUGGUA ::::. ::::::::.::: tae-miR408 3 翻译 Translation TraesCS6A02G378500.1 936 否 No 31—441 812—832 CCCAGGGCGGCGGCAGUGCAG ::::::.: :::::::::: 3.5 裂解 Cleavage TraesCS4B02G320300.1 1104 否 No 25—408 622—642 UUCAAGGAGGAGGCGGAGCAG .:: :::.:::::.: :::: TraesCS4D02G316800.1 1104 否 No 25—408 622—642 UUCAAGGAGGAGGCGGAGCAG .:: :::.:::::.: :::: TraesCS5A02G491700.1 1047 否 No 49—429 622—642 UUCAAGGAGGAGGCGGAGCAG .:: :::.:::::.: :::: tae-miR9780 3 翻译 Translation TraesCSU02G120000.1 1239 否 No 22—450 136—156 GACGCGUACGCCGCCGACCCG : ::::: :. :::::::::: 3.5 裂解 Cleavage TraesCS6A02G065600.1 1227 否 No 22—444 136—156 GACGCGUACGGCGCAGAUCCG : ::::: :.:::: ::.::: 翻译 Translation TraesCSU02G119900.1 1284 否 No 22—450 136—156 GACGCGUACGCCGCCGAUCCG : ::::: :. ::::::.::: tae-miR9676-5p 2.5 裂解 Cleavage TraesCS5B02G480900.1 900 是 Yes 58—351 755—776 ACCUCAGCUACGAUGACAUCCA : ::.::::::::::::: TraesCS5B02G480900.2 984 是 Yes 58—435 839—860 ACCUCAGCUACGAUGACAUCCA : ::.::::::::::::: tae-miR171a 3.5 裂解 Cleavage TraesCS4D02G175700.1 1074 否 No 28—408 747—767 GCUGAUGGCACGGCACGAUCA : :. ::::::::: :.:::: tae-miR444a 3.5 翻译 Translation TraesCS7A02G349500.1 1107 否 No 31—420 1011—1031 GGAGCAACUUGGAGGCAGCAG : :::::.: :::::::::. tae-miR444b 3.5 翻译 Translation TraesCS7A02G349500.1 1107 否 No 31—420 1011—1031 GGAGCAACUUGGAGGCAGCAG : :::::.: :::::::::. tae-miR9677a 3.5 裂解 Cleavage TraesCS1A02G276000.1 774 否 No 13—405 296—317 UGGUCUACUCCGCCGGCGGCCA .::::::.::..::::::