小麦TaNAC基因基于可变剪切和microRNA的转录后调控分析

吕士凯,马小龙,张敏,邓平川,陈春环,张宏,刘新伦,吉万全

Post-transcriptional Regulation of TaNAC Genes by Alternative Splicing and MicroRNA in Common Wheat (Triticum aestivum L.)

LÜ ShiKai,MA XiaoLong,ZHANG Min,DENG PingChuan,CHEN ChunHuan,ZHANG Hong,LIU XinLun,JI WanQuan

表1 TaNAC可变剪切结构变异转录本的克隆引物序列

Table 1 The primer sequences of TaNAC structural variation transcripts with alternative splicing in the gene cloning experiment

分组 Groups TaNAC基因 TaNAC genes 正向引物序列 Forward primer sequences (5′-3′) 反向引物序列 Reversed primer sequences (5′-3′) 1 TaNAC_NTL5_7B ATGACCCACCCTTCCTCGTC TTACTTGCCGTAGATGCACAG 2 TaNAC008_3A ATGGAAACTCTACGTGACATGGCT CTACAAACTGATCCCACCACGGT 3 TaNAC013_3A ATGACATGGTGCAATAGCTTC TCAGGGACCAAAGCCCGTC 4 TaNAC017_5A ATGGAGACCCCGCCGCCG TCATGTAGAGGACTTCCATAAC 5 TaNAC011_7D ATGGACGACGGTGCAACG TCACCCATGATGATCCTGGT 6 TaNAC017_5D ATGGAGACCCCGCCGCCG CTTTGTGCATCATCTTGTCATG 7 TaNAC024_3D ATGAACAGGGGTCACATCAGC CTATCCATGATGATCTTGGTTGTC 8 TaNAC039_6B ATGGACACTTTCTCCCATGTTC CTATTTCCACAGATGAGCCGAG 9 TaNAC073_3B ATGGACAAGGGTCACCCAGGGTAC CTAGTACTGGTAAGGCCATTGCAC 10 TaNAC092_2A TCCAGACGGCAAATTCCAG TCAGTAGCCCCACGGCGC 11 TaNAC092_2D ATGGAGCACAGCGAGCAGG TCAGTAGCCCCACGGCGC 12 TaNAC024_3A ATGAACAGGGGTCACATCAGC CTATGAGATCTCCCGGTTCTTCTC 13 TaNAC092_2B ATGGAGCACAGCGAGCAGG AGCTTGCTTGGTCAGTAGCC