干扰TP53INP2抑制牛成肌细胞分化

杜嘉伟,杜鑫泽,杨昕冉,宋贵兵,赵慧,昝林森,王洪宝

Interference in TP53INP2 Gene Inhibits the Differentiation of Bovine Myoblasts

DU JiaWei,DU XinZe,YANG XinRan,SONG GuiBing,ZHAO Hui,ZAN LinSen,WANG HongBao

表1 实时定量PCR引物序列

Table 1 Real-time PCR primer sequence

基因名称 Gene name GenBank号 GenBank No. 引物名称 Primer name 引物序列 (5′—3′） Primer sequence (5′--3′） 产物长度 Product length (bp) TP53INP2 XM_003586843.5 TP53INP2-F GCGGCTGTAGACTCAAAG 130 TP53INP2-R GTTATGAGGCGGAGTGTC GAPDH NM_001034034.2 GAPDH -F AGTTCAACGGCACAGTCAAGG 124 GAPDH -R ACCACATACTCAGCACCAGCA MYOD NM_001040478.2 MYOD-F AACCCCAACCCGATTTACC 196 MYOD-R CACAACAGTTCCTTCGCCTCT MYOG NM_001111325.1 MYOG-F GGCGTGTAAGGTGTGTAAG 85 MYOG-R CTTCTTGAGTCTGCGCTTCT MYH3 NM_001101835.1 MYH3-F TGAACGCCCTCTCCAAATCC 101 MYH3-R AATGAAGTGCTGTCTCGGCA