外泌体microRNA在猪成熟和闭锁卵泡中的表达差异及功能分析

陈慧芳,黄绮亮,胡智超,潘晓婷,吴志胜,白银山

Expression Differences and Functional Analysis of Exosomes microRNA in Porcine Mature and Atretic Follicles

CHEN HuiFang,HUANG QiLiang,HU ZhiChao,PAN XiaoTing,WU ZhiSheng,BAI YinShan

图1 外泌体分离检测与miRNA测序 A：闭锁卵泡的形态观察结果;B：成熟卵泡的形态观察结果;C：mffEXs粒径分析结果;D：affEXs粒径分析结果;E：Westem blot检测结果;F：miRNA的序列长度分布;G：mffEXs和affEXs中miRNA的表达差异结果

Fig. 1 Isolation and detection of exosomes and sequencing of miRNA A: Morphological observation of atretic follicles; B: Morphological observation of mature follicles; C: Particle size analysis results of mffexs; D: Particle size analysis results of affexs; E: Results of Westem blot analysis; F: Sequence length distribution of miRNA; G: Differential expression of miRNA in mffEXs and affEXs