外泌体microRNA在猪成熟和闭锁卵泡中的表达差异及功能分析

陈慧芳,黄绮亮,胡智超,潘晓婷,吴志胜,白银山

Expression Differences and Functional Analysis of Exosomes microRNA in Porcine Mature and Atretic Follicles

CHEN HuiFang,HUANG QiLiang,HU ZhiChao,PAN XiaoTing,WU ZhiSheng,BAI YinShan

表2 主要信号通路中miRNA调控靶基因信息表

Table 2 Information table of miRNA regulatory target genes in main significant pathways

信号通路 Signaling pathways 靶基因 Target gene list Ras信号通路 Ras signaling pathway (ssc04014) AKT2 (miR-148b-5p), CALM1 (miR-143-3p), IGF1 (let-7a), INS (miR-424-5p), KRAS (miR-181a), MAP2K1 (miR-1271-5p), MAPK10 (miR-148a-3p), PIK3CB (miR-126-5p), PRKACB (miR-146b), RAF1 (miR-125b) cAMP信号通路 cAMP signaling pathway (ssc04024) ADCY7 (miR-125b), AKT2 (miR-148b-5p), BRAF (let-7a), CALM1 (miR-130a), CAMK2A (miR-10a-5p), CAMK2G (miR-371-5p), GNAI2 (miR-124a), MAPK10 (miR-1), PDE3B (miR-126-5p), PIK3CG (miR-30a-3p), PPP1CC (miR-1343), PRKACB (miR-181a), RAF1 (miR-424-5) P53信号通路 P53 signaling pathway (ssc04115) CCNE2 (miR-1), IGF1 (miR-133a-3p), CDK1 (let-7a), CCNB (miR-9791-3p), P53 (miR-21), P53I3 (ssc-miR-7-5p) MAPK信号通路 MAPK signaling pathway (ssc04010) AKT2 (miR-10a-3p), BRAF (miR-18a), CDC25B (miR-146a-3p), KRAS (miR-181d-5p), MAPK10 (miR-199b-3p), MAPK8 (miR-199a-3p), PPP3CB (miR-202-3p), PRKACB (miR-143-5p), RAF1 (miR-424-5p), RPS6KA3 (miR-125a), P53 (miR-21) 卵母细胞减数分裂 Oocyte meiosis (ssc04114) ADCY7 (miR-125b), ANAPC1 (miR-326), AR (miR-124a), AURKA (miR-125a), BTRC (miR-1271-5p), CALM1 (miR-181b), CAMK2G (miR-371-5p), CCNE2 (miR-140-3p), CDC26 (miR-218), CDK1 (miR-143-3p), CPEB3 (miR-199b-3p), ESPL1 (miR-141), FBXO11 (miR-129a-3p), IGF1 (miR-133a-3p), ITPR1 (miR-200b), MAP2K1 (miR-143-5p), MAPK12 (miR-125b), MOS (miR-155-3p), PGR (miR-101), PKMYT1 (miR-106a) PLCZ (miR-124a), PPP3CD (miR-199a), PRKACB (miR-143-5p), PTTG1 (miR-1224), RBX (miR-218-3p), REC8 (miR-199b-3p), RPS6KA3 (let-7a), SGOL1 (miR-126-5p), SKP1 (miR-222), SMC1A (miR-128), SMC 3 (miR-9820-5P), STAG3 (miR-27b-3p), YWHAZ (miR-1) 泛素介导的蛋白水解作用 Ubiquitin mediated proteolysis (ssc04120) IGF1 (miR-133a-3p), BTRC (miR-1), FZR1 (miR-874), CDC27 (miR-1271-5), FBXW11 (miR-671-5p), RBX1 (miR-1224), SKP1 (miR-148a-3p) 细胞周期 Cell cycle (ssc04110） IGF1 (miR-133a-3p), BUB1 (miR-338), CCNE (miR-140-3p), CDC26 (miR-143-5p), CDK1 (let-7a), FZR1 (miR-138), MAD1L1 (miR-199b-5p), PKMYK1 (miR-106a), PLK1 (miR-18a), SMC1A (miR-132), SMC (miR-9820-5p), YWHAZ (miR-1), P53 (miR-21)