窖藏和冷藏条件下鸭梨挥发性物质及其相关基因表达分析

岳盈肖,何近刚,赵江丽,闫子茹,程玉豆,武肖琦,王永霞,关军锋

Comparison Analysis on Volatile Compound and Related Gene Expression in Yali Pear During Cellar and Cold Storage Condition

YUE YingXiao,HE JinGang,ZHAO JiangLi,YAN ZiRu,CHENG YuDou,WU XiaoQi,WANG YongXia,GUAN JunFeng

表5 窖藏、冷藏鸭梨果肉中挥发性成分变化

Table 5 Changes of volatile components in the flesh of Yali pear during cellar and cold storage (μg·kg-1)

种类 Kind 化合物名称 Compound name 贮藏初期 Initial storage 窖藏时间（月）Cellar storage time (Month) 冷藏时间（月）Cold storage time (Month) 1 2 3 1 2 3 醛类 Aldehydes 己醛 Hexanal 13.77±4.63a - - - 17.43±0.66a 15.82±7.13a 9.59±0.60a 2-已烯醛 2-Hexenal 0.60±0.29c - - - 6.48±0.18a 2.43±1.00b 3.48±0.93b (E)-2-辛烯醛 (E)-2-Octenal 0.59±0.02c 3.27±0.75b 5.95±0.63a 6.03±0.12a 2.93±0.24b 2.38±0.56b 3.09±0.38b 壬醛 Nonanal 2.72±0.34c 7.76±1.73bc 12.26±5.68a 9.27±0.47b 8.17±0.79ab 5.50±0.57bc 4.14±0.66bc (E)-2-壬烯醛 (E)-2-Nonenal 0.47±0.10d 3.59±1.19bc 5.75±1.57a 5.81±0.52a 4.55±0.14ab 3.41±0.79bc 1.97±0.30cd 癸醛 Decanal 0.78±0.05d 2.69±0.53bcd 8.72±2.97a 5.61±0.81b 4.26±0.63bc 3.04±0.58bcd 2.49±0.99cd (E)-2-癸醛 (E)- 2-Decenal 0.12±0.02c 2.47±0.22a - - 0.52±0.07b 0.48±0.05b 0.48±0.11b (E, E)-2,4-壬二烯醛(E, E)-2,4-Nonadienal - - - - 0.12±0.01 - - 庚醛 Heptanal - - - - 0.54±0.06 - - 2-庚烯醛 2-Heptenal - - - - 1.85±0.11 - - 总量 Total 19.04 19.78 32.69 26.72 46.84 33.05 25.23 酯类 Esters 丁酸乙酯 Butanoic acid ethyl ester - 345.70±150.45b 1164.28±476.33a 339.46±81.35b - - - 乙酸丁酯 Acetic acid butyl ester - - - - - 6.95±2.99 1.95±0.48 2-甲基丁酸乙酯 2-Methyl-butanoic acid ethyl ester - 58.71±21.63b 179.79±58.74a 70.37±13.25b - - - (E)-2-甲基-2-丁烯酸乙酯(E)-2-Methyl-2-butenoic acid ethyl ester - 9.67±1.11ab 15.98±5.21a 6.50±0.23c - - - 戊酸乙酯 Pentanoic acid ethyl ester - 7.64±2.13b 29.56±7.90a 14.31±2.36b - - - 己酸乙酯 Hexanoic acid ethyl ester - 561.10±113.48c 3681.45±480.26a 2763.89±96.90b - - - 2-己烯酸乙酯 2-Hexenoic acid ethyl ester - 18.85±3.80c 223.67±10.45a 143.88±5.27b - - - 庚酸乙酯 Heptanoic acid ethyl ester - 8.75±0.36b 62.29±6.15a 59.06±1.56a - - - 辛酸乙酯 Octanoic acid ethyl ester 0.17±0.04 b 7.50±1.55b 135.59±34.42a 159.95±8.23a 0.24±0.04b - - (E)-2-辛烯酸乙酯 (E)-2-Octenoate-ethyl ester - 3.53±0.93b 120.23±31.97a 134.06±5.49a - - - 4-辛烯酸乙酯 4-Octenoic acid ethyl ester - - 3.33±1.00 6.39±0.43 - - - (E,Z)-2,4-癸二烯酸乙酯(E,Z)-2,4-Decadienoic acid ethyl ester - 2.42±0.32c 142.47±7.18b 186.42±13.75a - - - 3-羟基己酸乙酯3-Hydroxy-hexanoic acid ethyl ester - - 17.84±5.09 17.98±0.83 - - - 3-甲硫基-2-丙烯酸乙酯3-(Methylthio)-(E)-2-propenoate ethyl ester - 25.46±0.69c 80.42±6.86a 32.55±3.56b - - - 苯乙酸乙酯 Benzeneacetic acid ethyl ester - 0.61±0.03c 1.94±0.13b 2.59±0.24a - - - 种类 Kind 化合物名称 Compound name 贮藏初期 Initial storage 窖藏时间（月）Cellar storage time (Month) 冷藏时间（月）Cold storage time (Month) 1 2 3 1 2 3 己酸丙酯 Hexanoic acid propyl ester - - 1.87±0.57 1.47±0.06 - - - 乙酸己酯 Acetic acid hexyl ester 3.02±0.18 f 104.95±7.55b 202.30±4.81a 38.98±2.12c 8.15±0.26ef 24.26±1.49d 12.82±0.67e 乙酸庚酯 Acetic acid heptyl ester - 4.33±1.02a 6.01±1.35a 1.21±0.20b - 0.41±0.09b - 乙酸辛酯 Acetic acid octyl ester - 19.35±4.09b 48.95±12.59a 11.92±0.93b - - - 2,2,4-三甲基-1,3-戊二醇二异丁酸酯 2,2,4-Trimethyl-1,3-pentanediol diisobutyrate 0.11±0.04c 9.48±1.17bc 27.55±8.56a 19.65±13.23 ab 6.66±1.89 bc 7.16±4.12 bc 4.23±0.87c 己二酸二异丁酯 Hexanedioic acid di-isobutyl ester - - 3.51±1.56a 3.75±2.61a 0.40±0.19b - - 总量 Total 3.31 1188.05 6149.01 4014.39 15.44 38.77 19.00 烷烃类 Alkanes 癸烷 Decane 91.53±75.84a - - - 118.79±7.39a 164.08±67.55a 86.19±24.54a 十四烷 Tetradecane 0.32±0.04 - - - - - - 十六烷 Hexadecane 0.13±0.03 - - - - - - 总量 Total 91.97 0.00 0.00 118.79 164.08 86.19 醇类 Alcohols 2-乙基己醇 2-Ethyl hexanol 0.09±0.08b 0.98±0.37a - - 1.03±0.23a 1.01±0.59a 0.56±0.18ab 正辛醇 Octanol 0.19±0.06d 9.56±1.73b 26.29±1.45a 6.63±0.75c 0.28±0.04d 0.95±0.06d 0.25±0.08d 正癸醇 Decanol - 2.62±0.10c 79.54±5.92a 19.52±2.12b - - - (Z)-4-癸烯醇 (Z)-4-Decenol - 3.75±0.15c 45.83±1.36a 27.86±1.26b - - - 2,4-癸二烯醇 2,4-Decadienol - 1.85±0.29 2.07±0.23 - - - - 总量 Total 0.28 18.76 153.73 54.01 1.31 1.96 0.81 萜类 Terpenoids (Z,E)-金合欢烯 (Z,E)-3,7,11-Trimethyl-1,3,6,10-Dodecatetraene - 5.68±0.60c 34.83±7.47a 18.50±4.74b 0.15±0.05c 1.36±0.48c 0.49±0.14c α-法尼烯α-Farnesene - 192.30±9.99b 513.13±117.75a 470.72±94.19a 3.90±0.76c 32.67±14.62c 14.34±4.48c 总量 Total 0.00 197.99 547.96 489.22 4.05 34.03 14.83 其他 Others 乙酸-2-甲氧基-4-(2-丙烯基)苯酚Acetate-2-methoxy-4-(2-propenyl)-phenol - - - - - - 0.19±0.01 2-烯丙基苯酚 2-Allylphenol - - - - - 1.12±0.12 0.41±0.04 (Z)-6,10-二甲基-5,9-十一碳二烯-2-酮(Z)-6,10-Dimethyl-5,9-Undecadien-2-one 0.17±0.03a - - - 0.23±0.01a 0.46±0.22a 0.46±0.19a 6-甲基-5-庚烯-2-酮6-Methyl-5-Hepten-2-one - 1.14±0.24b 3.59±1.28a 4.45±0.27a - - - 总量 Total 0.17 1.14 3.59 4.45 0.23 1.57 1.06 挥发性成分总量 Total amount of volatile components 114.77 1425.71 6886.97 4588.78 186.65 273.46 147.12