窖藏和冷藏条件下鸭梨挥发性物质及其相关基因表达分析
岳盈肖,何近刚,赵江丽,闫子茹,程玉豆,武肖琦,王永霞,关军锋

Comparison Analysis on Volatile Compound and Related Gene Expression in Yali Pear During Cellar and Cold Storage Condition
YUE YingXiao,HE JinGang,ZHAO JiangLi,YAN ZiRu,CHENG YuDou,WU XiaoQi,WANG YongXia,GUAN JunFeng
表4 窖藏、冷藏鸭梨果皮中挥发性成分变化
Table 4 Changes of volatile components in the peel of Yali pear during cellar and cold storage (μg·kg-1)
种类
Kind
化合物名称
Compound name
贮藏初期
Initial storage
窖藏时间(月)Cellar storage time (Month) 冷藏时间(月)Cold storage time (Month)
1 2 3 1 2 3
醛类
Aldehyde
己醛 Hexanal 610.83±176.46a 571.58±255.42ab - - 473.64±35.71ab 381.78±44.51ab 240.45±19.71b
(E)-2-己烯醛 (E)-2-Hexenal 128.13±29.39d 401.05±60.10a 352.44±64.79ab 435.08±76.48a 397.47±35.38a 265.22±26.89bc 234.52±11.08c
壬醛 Nonanal 20.93±6.37c 54.78±5.43a 32.92±1.63b 23.42±4.00c 32.81±3.86b 20.73±0.66c 17.48±0.96c
1,1-二甲氧基己烷 1,1-dimethoxyhexane 4.82±2.49b 23.69±5.92a 7.94±3.45b - 3.36±1.34b - -
癸醛 Decanal 1.63±0.46c 18.93±5.60b 29.01±3.99a 20.12±3.54b 3.09±0.92c 3.10±0.02c 3.16±1.34c
(E,Z)-2,4-癸二烯醛 (E,Z)- 2,4-Decadienal - 13.05±4.17a 3.98±0.89b 10.09±2.22ab - - -
总量 Total 766.34 1083.08 426.29 488.70 910.38 670.83 495.61
酯类
Esters
丁酸甲酯Butanoic acid methyl ester - - - - 17.09±2.59a 10.97±2.96a 11.49±4.56a
己酸甲酯 Hexanoic acid methyl ester - 14.80±1.46b 35.84±1.56a 29.13±6.36a - - -
辛酸甲酯 Octanoic acid methyl ester 2.77±0.87c 149.02±22.37b 402.74±61.97a 319.80±69.75a - 4.97±2.05c -
水杨酸甲酯 Salicylic acid methyl ester 3.66±0.30 - - - - - -
3-甲硫基-(E)-2-丙烯酸乙酯
3-(methylthio)-(E)-2-propenoate- ethyl ester
- 174.72±13.53a 158.19±20.90a 75.61±12.99b - - -
丁酸乙酯 Butanoic acid ethyl ester - 861.78±127.76b 2234.49±246.21a 1211.75±174.70b - - -
(E)-2-甲基-2-丁烯酸乙酯 (E)-2-Methyl-2-butenoic acid ethyl ester - 27.85±2.98a 26.73±5.05a 11.51±4.56b - - -
戊酸乙酯 Pentanoic acid ethyl ester - 26.85±2.19 51.50±5.19 - - - -
己酸乙酯 Hexanoic acid ethyl ester - 4615.33±100.96c 8448.46±307.96a 6463.16±710.28b - 86.30±29.03d -
2-己烯酸乙酯 2-Hexenoic acid ethyl ester - 91.16±1.61c 271.51±9.38a 171.49±20.07b - - -
庚酸乙酯 Heptanoic acid ethyl ester - 45.29±3.59b 95.29±7.35a 85.59±14.12a - - -
辛酸乙酯 Octanoic acid ethyl ester - 1774.77±279.96b 2746.27±345.26a 2411.39±412.61a 1.18±0.13c 15.35±0.73c -
4-辛烯酸乙酯 4-Octenoic acid ethyl ester - 15.49±2.21c 37.35±5.86b 67.77±12.05a - 2.36±0.48c -
癸酸乙酯 Decanoic acid ethyl ester - 45.03±8.91b 199.58±248.81a 251.87±29.21a - - -
(E)-2-癸烯酸乙酯 (E)-2-Decenoic acid ethyl ester - - 8.03±5.79 - - -
(E)-4-癸烯酸乙酯 (E)-4-Decenoic acid ethyl ester - - 35.05±6.68 41.69±12.71 - - -
(E,Z)-2,4-癸二烯酸乙酯 (E,Z)-2,4-Decadienoic acid ethyl ester - 80.09±11.23c 1123.28±114.87b 1482.82±208.68a - - -
苯甲酸乙酯 Benzoic acid ethyl ester - 6.21±1.18 - - - 3.48±0.15 -
苯乙酸乙酯 Benzene-acetic acid ethyl ester - - - 5.25±1.12 - - -
2,2,4-三甲基-1,3-戊二醇二异丁酸酯
2,2,4-Trimethyl-1,3-pentanediol di-isobutyrate
2.68±1.90b - - - 50.79±4.43a 32.39±20.10a 27.55±16.89ab
种类
Kind
化合物名称
Compound name
贮藏初期
Initial storage
窖藏时间(月)Cellar storage time (Month) 冷藏时间(月)Cold storage time (Month)
1 2 3 1 2 3
己二酸二异丁酯 Hexanedioic acid di-isobutyl ester - - - - - - 4.72±1.66
乙酸己酯 Acetic acid hexyl ester 19.51±3.21f 426.39±5.87a 285.42±9.13b 65.37±8.27e 79.11±6.79e 140.07±18.12c 101.87±8.44d
丁酸己酯 Butanoic acid hexyl ester - - - - - 5.73±1.34 -
乙酸庚酯 Acetic acid heptyl ester - 22.87±3.35 - - - - -
乙酸辛酯 Acetic acid octyl ester - 317.87±44.09a 179.21±22.90b 64.47±8.75c - 5.97±0.72d 1.88±0.40d
乙酸癸酯 Acetic acid decyl ester - - 5.54±0.70 - - - -
总量Total 28.62 8695.51 16336.46 12766.68 148.17 307.59 147.51
烷烃类
Alkanes
癸烷 Decane 1014.95±681.18a - - - 1191.52±414.35a 567.77±39.08a 631.00±134.43a
十一烷 Undecane 3.65±2.56 - - - - - -
十三烷 Tridecane - - - - - - 1.14±0.19
十四烷 Tetradecane 6.24±1.84 - - - 6.13±1.24 - -
十五烷 Pentadecane 6.15±2.34 - - - - - -
十六烷 Hexadecane 4.92±2.59 - - - - - -
二十烷 Eicosane 0.76±0.42b - - - 7.11±0.55b 24.96±6.46a 3.90±0.49b
总量 Total 1036.66 0.00 0.00 0.00 1204.75 592.73 636.04
醇类
Alcohols
正辛醇 1-Octanol 3.91±1.12d 206.41±12.57a 181.77±10.68b 91.63±9.57c - 8.38±1.00d -
正癸醇 1-Decanol - 143.13±18.99c 510.37±44.30a 257.50±22.23b - - -
2,4-癸二烯醇 2,4-Decadienol - 78.90±10.39a 57.93±9.67b 18.61±1.33c 2.47±0.49d 5.87±0.82d 1.85±0.05d
(Z)-4-癸烯-醇 (Z)-4-Decenol - 163.83±28.42c 671.34±52.60a 501.19±63.90b 3.41±0.91d 11.02±1.50d 4.95±0.54d
1,10-癸二醇 1,10-Decanediol - - - 25.25±0.59 - - -
总量 Total 3.91 592.27 1421.41 894.18 5.88 25.28 6.80
萜类
Terpenoids


(Z,E)-金合欢烯 (Z,E)-3,7,11-Trimethyl-1,3,6,10-dodecatetraene - - - - 81.19±4.60c 234.64±10.19a 100.92±8.77b
β-法尼烯 β-Farnesene - 18.08±2.96a 15.06±1.77a 17.205±3.90a - 5.09±0.17b 2.00±0.42b
α-法尼烯α-Farnesene 8.06±1.49e 21147.84±1920.87a 16616.83±1260.42b 16321.98±2382.14b 4685.73±547.41d 10898.67±1476.44c 4775.20±407.78d
总量Total 8.06 21165.91 16631.89 16339.19 4766.92 11138.40 4878.12
其他
Others
丁香酚2-Methoxy-3-(2-propenyl)-phenol - - - 8.01±2.80 - - -
4-烯丙基苯酚4-(2-Propenyl)-phenol - - - - 4.70±0.33c 27.93±1.92a 10.29±1.04b
6-甲基-5-庚烯-2-酮 6-Methyl- 5-hepten-2-one 2.91±1.20e 47.97±1.73c 71.86±4.34b 106.19±11.01a 7.51±0.70e 33.18±2.92d 33.43±2.44d
总量 Total 2.91 47.97 71.86 114.19 12.22 61.11 43.72
挥发性成分总量 Total amount of volatile components 1846.50 31584.73 34887.91 30602.95 7048.31 12795.93 6207.79