窖藏和冷藏条件下鸭梨挥发性物质及其相关基因表达分析

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Comparison Analysis on Volatile Compound and Related Gene Expression in Yali Pear During Cellar and Cold Storage Condition

YUE YingXiao,HE JinGang,ZHAO JiangLi,YAN ZiRu,CHENG YuDou,WU XiaoQi,WANG YongXia,GUAN JunFeng

表2 定量PCR引物序列

Table 2 Real-time PCR primer sequence

基因 Gene 序列号 Genbank ID 引物序列 Primer sequence PbACS1 XM_018643584.1 5′-GGGCACCACAATGACCAGA-3′ 5′-GTGAACTCGCTGCCAAAC-3′ PbACO2 XM_009357643.1 5′-GGTTCAAGGAAATGGTGGC-3′ 5′-AGCCTTCTTTAGATAGCCCTTC-3′ PbETR1a XM_009367968.2 5′-AGTCTAAGCAGCCTTTTGCACC-3′ 5′-TGCTGACCCCATTATCATCC-3′ PbETR1b XM_009350325.2 5′-GATCCTGGAGAATCATCAGAGC-3′ 5′-GCAGTTACAATGCAACCAAGC-3′ PbERS1 XM_009380469.1 5′-CGTTGCATTCATCGTCAAACTG-3′ 5′-TCGATGAACTTGCGCCAAATCG-3′ PbERS2 KF188466.1 5′-CGTTGCATTCATCGTCCAACTA-3′ 5′-TTCGACGAAGTTACTCCGAAAC-3′ PbEIN3 XM_009340977.2 5′-CCCGTGACATCTTCTTCCTG-3′ 5′-TGACTTTCTTTCGGCTCCC-3′ PbERF XM_009380720.2 5′-TTGAAAGAGTCCGCAAATCG-3′ 5′-CCAAGTAATCCGTTCCCAAA-3′ PbAAT1 XM_018649523.1 5′-TGGTGCCAAGGAGATGAGAGTC-3′ 5′-TTGTGGTGCTTTCCCCGTG-3′ PbADH2 KU507380.1 5′-CGGAAACTACAAGACTCGAA-3′ 5′-CGGATTATGCAACGAAGAC-3′ PbADH3 XM_009362449.1 5′-TGTGACCTCCTCAGGATAAA-3′ 5′-GCCAAGGGATTGATCTTAG-3′ PbADH5 XM_009379426.2 5′-CGCCGACATCAACAAAGCAT-3′ 5′-TGCAATACCCTCAAAATCCATGT-3′ PbHPL XM_009348862.2 5′-CGGTGTTCCGCACAAATA-3′ 5′-AAGCGCAGGTCCTCAAGTC-3′ PbLOX1 XM_009378388.2 5′-TGAGCCATTTGTGATAGCGG-3′ 5′-TGTGAGAACTTGTCGTGCGG-3′ PbLOX8 XM_009378384.2 5′-GCCACAAACAGGCAACTAA-3′ 5′-CTCGGTGAAATTCCAATCC-3′ PbAcitin2 GU830959 5′-GGACATTCAACCCCTCGTCT-3′ 5′-ATCCTTCTGACCCATACCAACC-3′