枯草芽孢杆菌NCD-2对棉花根系分泌物L-脯氨酸响应的转录-蛋白质组学联合分析

赵卫松,郭庆港,董丽红,王培培,苏振贺,张晓云,鹿秀云,李社增,马平

Transcriptome and Proteome Analysis of Bacillus subtilis NCD-2 Response to L-proline from Cotton Root Exudates

ZHAO WeiSong,GUO QingGang,DONG LiHong,WANG PeiPei,SU ZhenHe,ZHANG XiaoYun,LU XiuYun,LI SheZeng,MA Ping

图6 差异表达基因的RT-qPCR验证 1—13代表能量代谢（氨基酸、糖类等）相关的基因,分别为putC、rocA、ald、gapB、yerA、mmgD、asnO、acoC、yisZ、thrD、ggt、yjmD和yoaD;14—19代表ABC转运蛋白相关的基因,分别为scoB、glnQ、opuCC、opuCA、znuA和ssuB;20、21代表鞭毛组装相关的基因,分别为fliF和fliY;22、23代表抗生素合成相关的基因,分别为gcvPB和yodQ;24、25代表生物膜形成相关的基因,分别为epsA和epsC;26代表趋化作用相关的基因cheY

Fig. 6 RT-qPCR validation of DEGs 1-13 are genes related to energy metabolism (amino acids, sugars, etc.), representing putC, rocA, ald, gapB, yerA, mmgD, asnO, acoC, yisZ, thrD, ggt, yjmD and yoaD, respectively. 14-19 are genes related to ABC transporters, representing scoB, glnQ, opuCC, opuCA, znuA and ssuB, respectively. 20-21 are genes related to flagellar assembly, representing fliF and fliY, respectively. 22-23 are genes related to biosynthesis of antibiotics, representing gcvPB and yodQ, respectively. 24-25 are genes related to biofilm formation, representing epsA and epsC, respectively. 26 represents chemotaxis related gene cheY