枯草芽孢杆菌NCD-2对棉花根系分泌物L-脯氨酸响应的转录-蛋白质组学联合分析

赵卫松,郭庆港,董丽红,王培培,苏振贺,张晓云,鹿秀云,李社增,马平

Transcriptome and Proteome Analysis of Bacillus subtilis NCD-2 Response to L-proline from Cotton Root Exudates

ZHAO WeiSong,GUO QingGang,DONG LiHong,WANG PeiPei,SU ZhenHe,ZHANG XiaoYun,LU XiuYun,LI SheZeng,MA Ping

表1 引物及其碱基序列

Table 1 Primers and their base sequences

基因 Gene 序列Sequence (5′ to 3′) 基因 Gene 序列Sequence (5′ to 3′) fliF F ACCGAAAGCGGGAACTAC opuCC F GCAAGGAAGCGGAGAAAG R CATCAGGCGGCTCTACCA R AGCCGTAGGAATCAAACCA gInQ F TATCGGGATGGTGTTTCA gcvPB F TTATTTCCCGCTTAATGTTG R GTCAGCCTTGTCTGGGAT R TCTTCCGCCTCGTATCTG cheY F GGAGCACAAGCGGTAGAG putC F AATCGTTTCAATCAACCCAG R CTGAATGGCATCAATAAC R TATCGGCATCCGCCTCGT fliY F CAGACCGTATTCCTGATG rocA F TAGTTGAGCATCCGAAGAC R CACCGTTTCTTCTTCCTC R CAATTACCCGTTTGAGCC opuCA F CCAGCAGAACATCTCACTC yodQ F GGCAATACAGCCCTTCTT R GCGGATAACGGTCTAAATAC R GCCGCACTCTTCATCTAC znuA F GGGCTTTCACCTGACCAA ald F CCTCTTCTGACGCCAATG R CGAGCGTATCTGCGACCT R CAACGCCTCCTCCGATAA gapB F AAGAGGTTGTGGCTGGTG yerA F GAGAAGGCTGGAACTGGG R TTGCTTCGACGACTATGT R ATTGTAGGCGTCGATTGC mmgD F AATGGAAACGCTGGAACG asnO F TGTCGGATGTGCCTGTTT R TGTTTGCGGAAGGAGACC R CATTTGTGGTGCGTTGTG acoC F TGGACCAGGCGGACGAAT yisZ F AGGATCGGGACATGGTTA R TCGGGCAGCGATGACCTT R ATGAAATCGGGTGAAAGC thrD F ACTGATCGCCGCTTACTT ggt F ACACTGTCCAGGATTTCG R ACCGCTCCGTGAGAATGT R GGAGGAGGAGTAGTAGCG yjmD F CAGCGGAGGTGAAGAAGC yoaD F CCCGAACTTTCATTTGTC R GAGGGTAGCGAGCGGATT R CTCCATCCTTCAGCCACT epsA F TCGAATCTCAGTGACATCCA epsC F AATCCAGAAGAGGCGGTCAA R AGATAGGTGCAATTCCGC R GCCGAAGCGAACAGCAAC ssuB F ATTATCAATCGCACCAGG scoB F TTGTCGCAAATGAGATACCC R CAACAGTCAGCCAAGGAA R CGTCTTCCGTTCCTTCCA gyrB F GAAGCACGGACAATCACC R TCCAAAGCACTCTTACGG