棉花真叶原生质体分离及瞬时表达体系的优化

李青,鱼海鹏,张子豪,孙正文,张艳,张冬梅,王省芬,马峙英,阎媛媛

Optimization of Cotton Mesophyll Protoplast Transient Expression System

LI Qing,YU HaiPeng,ZHANG ZiHao,SUN ZhengWen,ZHANG Yan,ZHANG DongMei,WANG XingFen,MA ZhiYing,YAN YuanYuan

图4 转化条件的优化 A、C、D：培养液类型、甘露醇和PEG浓度对转化效率的影响;B：荧光显微镜下的转化结果

Fig. 4 Optimization of transformation conditions A, C and D: Effect of protoplast culture solution, mannitol concentration and PEG concentration on transformation efficiency; B: Protoplasts after transformation under fluorescence microscope