棉花真叶原生质体分离及瞬时表达体系的优化

李青,鱼海鹏,张子豪,孙正文,张艳,张冬梅,王省芬,马峙英,阎媛媛

Optimization of Cotton Mesophyll Protoplast Transient Expression System

LI Qing,YU HaiPeng,ZHANG ZiHao,SUN ZhengWen,ZHANG Yan,ZHANG DongMei,WANG XingFen,MA ZhiYing,YAN YuanYuan

图3 酶解时间对原生质体分离的影响 A：不同酶解时间下原生质体产量;B—E：酶解7—12 h得到的原生质体;F—G：原生质体活力检测

Fig. 3 Effect of digestion time on protoplast isolation A: Protoplast yield after different digestion time; B-E: Isolated protoplasts after 7-12 h digestion; F-G: Detection of protoplast viability