棉花真叶原生质体分离及瞬时表达体系的优化

李青,鱼海鹏,张子豪,孙正文,张艳,张冬梅,王省芬,马峙英,阎媛媛

Optimization of Cotton Mesophyll Protoplast Transient Expression System

LI Qing,YU HaiPeng,ZHANG ZiHao,SUN ZhengWen,ZHANG Yan,ZHANG DongMei,WANG XingFen,MA ZhiYing,YAN YuanYuan

图2 叶龄对原生质体分离的影响 A：取样真叶;B：不同叶龄原生质体产量;C：老叶原生质体;D：幼叶原生质体

Fig. 2 Effect of leave developmental stage on protoplast isolation A: True leaves for protoplast isolation; B: Protoplast yields using the true leaves at different developmental stages; C: Protoplasts produced from elder leaves; D: Protoplasts produced from newly flattened young leaves