棉花真叶原生质体分离及瞬时表达体系的优化

李青,鱼海鹏,张子豪,孙正文,张艳,张冬梅,王省芬,马峙英,阎媛媛

Optimization of Cotton Mesophyll Protoplast Transient Expression System

LI Qing,YU HaiPeng,ZHANG ZiHao,SUN ZhengWen,ZHANG Yan,ZHANG DongMei,WANG XingFen,MA ZhiYing,YAN YuanYuan

图1 不同浓度CaCl2和甘露醇对原生质体分离的影响 A：不同浓度CaCl2酶解液酶解效果;B：甘露醇对原生质体得率的影响（不同小写字母表示在P<0.05水平差异显著。下同）;C：甘露醇对原生质体活力的影响

Fig. 1 Effect of CaCl2 and mannitol concentration on protoplast isolation A: Digestion effect of enzyme buffer with different CaCl2 concentration; B: Effect of mannitol concentration on protoplast yield (different letters show significant difference at P<0.05. The same as below); C: Effect of mannitol on protoplast viability