过表达ZmIBH1-1提高玉米苗期抗旱性

朱芳芳,董亚辉,任真真,王志勇,苏慧慧,库丽霞,陈彦惠

Over-expression of ZmIBH1-1 to Improve Drought Resistance in Maize Seedlings

ZHU FangFang,DONG YaHui,REN ZhenZhen,WANG ZhiYong,SU HuiHui,KU LiXia,CHEN YanHui

图7 ZmIBH1-1对靶基因的调控 A：35S启动子启动海肾荧光素酶（REN）作为内参,基因启动子启动报告基因萤火虫荧光素酶（LUC）基因,ZmIBH1-1作为效应因子,空载体为对照,在本氏烟草中瞬时表达。B—C：LUC/REN的比值代表启动子的活性,每个试验重复3次（n=3,±SD,**P<0.01）。NC：阴性对照

Fig. 7 Regulation of target genes by ZmIBH1-1 A: The 35S: REN-Pro gene LUC reporter constructs were transiently expressed in N.benthamiana leaves together with control vector or 35S:ZmIBH1-1 effector, respectively. The expression level of REN was used an internal control; B-C: The LUC/REN ratio represents the relative activity of promoters. Data are values of three independent experiments. Significant differences from the corresponding control values (n=3, ±SD, **P<0.01). NC: Negative control