基于SodC单克隆抗体的胞内劳森菌IPMA抗原检测方法的建立及应用

李敏雪,李剑男,周红,肖宁,蔺辉星,马喆,范红结

Establishment and Preliminary Application of Lawsonia intracellularis IPMA Antigen Detection Method Based on SodC Monoclonal Antibody

LI MinXue,LI JianNan,ZHOU Hong,XIAO Ning,LIN HuiXing,MA Zhe,FAN HongJie

图1 重组蛋白SodC的SDS-PAGE分析 M：蛋白分子量标准;1：pGex-6p-1空载体质粒;2：未诱导的重组表达质粒;3：诱导表达后的全菌液;4：诱导表达后的上清;5：诱导表达后的包涵体;6：纯化后的SodC蛋白

Fig. 1 SDS-PAGE analysis of recombinant protein SodC M: Molecular weight standard of protein; 1: pGex-6p-1 no-load particle; 2: Uninduced recombinant expression plasmid; 3: Whole bacterial liquid induced and expressed; 4: Supernatant after induced expression; 5: Inclusion body after induced expression; 6: Purified SodC protein