基于SodC单克隆抗体的胞内劳森菌IPMA抗原检测方法的建立及应用

李敏雪,李剑男,周红,肖宁,蔺辉星,马喆,范红结

Establishment and Preliminary Application of Lawsonia intracellularis IPMA Antigen Detection Method Based on SodC Monoclonal Antibody

LI MinXue,LI JianNan,ZHOU Hong,XIAO Ning,LIN HuiXing,MA Zhe,FAN HongJie

表1 IPMA对临床样品的检测

Table 1 Detection of clinical samples by IPMA

猪场 Pig farm 样品数 sample IPMA PCR 阳性 Positive 阴性 Negative 检出率 Detection rate (%) 阳性 Positive 阴性 Negative 检出率 Detection rate (%) 泰州Taizhou 61 40 21 65.6 34 27 44.3 镇江Zhenjiang 44 30 14 68.1 26 18 59.1 盐城Yancheng 41 22 19 53.7 22 19 53.7 总计Total 146 92 54 63.0 82 64 56.2