山羊TNNT3基因可变剪切及其对骨骼肌细胞分化的作用

陈媛,蔡禾,李利,王林杰,仲涛,张红平

Alternative Splicing of TNNT3 and Its Effect on the Differentiation of MuSCs in Goat

CHEN Yuan,CAI He,LI Li,WANG LinJie,ZHONG Tao,ZHANG HongPing

图5 山羊TNNT3_3体外转录及对细胞分化的作用 A：TNNT3_3体外转录载体构建;B：TNNT3_3体外转录蛋白免疫印迹结果;C：TNNT3_3过表达后增殖GM/分化DM时期标志基因mRNA水平;D：分化7 d后MyHC免疫荧光结果;M1：DNA marker DL 2000 bp;M2：DNA marker DL 5000 bp;M3：蛋白marker

Fig. 5 Transcription in vitro and overexpression of TNNT3_3 and its effect on the differentiation in goat MuSCs A: Construction of TNNT3_3 transcriptional vector in vitro; B: Western blot results of TNNT3_3 transcriptional protein in vitro; C: mRNA levels of marker genes in the proliferative/ differentiation stage of MuSCs treated with TNNT3_3 vector; D: MyHC immunofluorescence results after differentiation for 7 days; M1：DNA marker DL 2000 bp;M2：DNA marker DL 5000 bp;M3：Protein marker