山羊TNNT3基因可变剪切及其对骨骼肌细胞分化的作用

陈媛,蔡禾,李利,王林杰,仲涛,张红平

Alternative Splicing of TNNT3 and Its Effect on the Differentiation of MuSCs in Goat

CHEN Yuan,CAI He,LI Li,WANG LinJie,ZHONG Tao,ZHANG HongPing

图2 山羊TNNT3基因PCR扩增、克隆及不同可变剪切体序列与山羊全基因组数据库比对 A：TNNT3 CDS区全长PCR产物(P1:835bp);B：TNNT3可变剪切体单克隆菌液PCR产物;C：TNNT3可变剪切体差异片段（exon4-8：193-253 bp）qPCR产物;M1：DNA marker DL 2000 bp;M2: DNA marker DL 5000 bp;D：TNNT3不同可变剪切体序列与山羊全基因组数据库比对结果,红色方框是发生可变剪切的外显子位点;E：山羊TNNT3不同可变剪切体的氨基酸序列对比;绿色方框：骨骼肌中最先被降解的产物[18];红色方框：TNNT3_3的外显子Exon 9—11重复,增加了46个氨基酸残基;蓝色方框：互斥表达的外显子16/17编码的氨基酸[25];F：TNNT3可变剪切体结构域预测

Fig. 2 PCR amplification, cloning results of TNNT3 gene in goats and comparison results of different alternative transcripts sequences with the goat genome database A: PCR products of TNNT3 full-length CDS region (835bp); B: PCR products of TNNT3 isoforms from monoclonal bacteria solution; C: qPCR products with TNNT3 transcripts at alternative fragment (Exon4-8: 193bp-253bp ; M1: DNA Marker DL 2000 bp; M2: DNA Marker DL 5000 bp; D: Comparison results of different alternative transcripts sequences with the Goat genome database; The red box is an exon site that can be alternative splicing; E: Amino acid sequences of different alternative transcripts of TNNT3 in goats; Green box: The first degraded product in skeletal muscle[18]; Red box: the Exon 9-11 of TNNT3_3 is repeated with 46 amino acid; Blue box: Exon 16 and 17 coding amino acid are alternatively spliced in a mutually exclusive manner[25]; F: TNNT3 alternative transcripts domain prediction