控制桃粘/离核PG基因的BAC克隆筛选与序列分析

孟君仁,牛良,邓丽,潘磊,鲁振华,崔国朝,王志强,曾文芳

Screening and Sequence Analysis of BAC Clone Contained PG Gene Controlling Clingstone/Freestone Characteristic of Peach

MENG JunRen,NIU Liang,DENG Li,PAN Lei,LU ZhenHua,CUI GuoChao,WANG ZhiQiang,ZENG WenFang

图6 PCR验证离/粘核桃 M：DL 2000 DNA Marker。1—10：以离核桃为模板,分别为春雪、枣油桃、中油22号、中桃6号、中桃24号、新中南12-14、新中南12-36、新中南12-44、新中南12-54、新中南13-10;11—20：以粘核桃为模板,分别为新中南12-55、新中南12-59、新中南12-60、新中南13-4、新中南13-14、87-7-1、中油8号、中油13号、中桃5号、中油桃4号;21：灭菌水

Fig. 6 PCR confirmed positive clones freestone/clingstone peach M: DL 2000 DNA Marker. 1-10: Freestone peach. Chunxue, Zaoyoutao, Zhongyou 22, Zhongtao 6, Xinzhongnan12-14, Xinzhongnan12-36, Xinzhongnan12-44, Xinzhongnan12-54, Xinzhongnan13-10, respectively; 11-20: Clingstone peach. Xinzhongnan12-55, Xinzhongnan12-59, Xinzhongnan12-60, Xinzhongnan13-4, Xinzhongnan13-14, 87-7-1, Zhongyou 8, Zhongyou 13, Zhongtao 5, Zhongyoutao 4, respectively; 21: The negative control uses sterilized water as the template