控制桃粘/离核PG基因的BAC克隆筛选与序列分析

孟君仁,牛良,邓丽,潘磊,鲁振华,崔国朝,王志强,曾文芳

Screening and Sequence Analysis of BAC Clone Contained PG Gene Controlling Clingstone/Freestone Characteristic of Peach

MENG JunRen,NIU Liang,DENG Li,PAN Lei,LU ZhenHua,CUI GuoChao,WANG ZhiQiang,ZENG WenFang

图3 PCR验证阳性克隆和质粒提取的琼脂糖检测 A：M：DL 2000 DNA Marker;泳道1—14：以BAC克隆46-B-1-14为模板,阳性对照以粘核溶质桃‘黄金蜜桃3号’为模板,阴性对照以灭菌水为模板。B：M：TSINGKE 1.5 kb Marker,BAC：提取的质粒

Fig. 3 Agarose assays for PCR confirmed positive clones and the BAC clone plasmid A: M: DL 2000 DNA Marker; Lane 1-14: BAC clones 46-B-1-14 as template, and the positive control uses the clingstone with melting flesh peach Huangjinmi 3 as the template, while the negative control uses sterilized water as the template. B: M: TSINGKE 1.5 kb Marker, BAC: Plasmid extracted