番茄SlN-like的克隆、表达与抗病毒功能

刘昌云,李欣羽,田绍锐,王靖,裴悦宏,马小舟,樊光进,汪代斌,孙现超

Cloning, Expression and Anti-Virus Function Analysis of Solanum lycopersicum SlN-like

LIU ChangYun,LI XinYu,TIAN ShaoRui,WANG Jing,PEI YueHong,MA XiaoZhou,FAN GuangJin,WANG DaiBin,SUN XianChao

表1 本研究所用到的引物

Table 1 Primers used in this study

引物名称 Primer name 引物序列F Primer sequence F (5′-3′) 引物序列R Primer sequence R (5′-3′) PCR P1 ATGAATCAGGAAAGTTCAGTGC CGGCTAATGCATCTAACTGTTC P2 GAACAGTTAGATGCATTAGCCG CATGCCAATCAAGCCACCTC P3 TGGCACTGACAAGATCGAAG CATCGAGAATCAATCCCTCC P4 GGAGGGATTGATTCTCGATG TCAATTCTCATACACAAGACG qPCR SlN-like ACTTTCCTAGCCCACGTACC ATACACAAGACGCACTCCA TMV MP TTAGGTTCCCTGACGGTGAC ACCGTTGCGTCGTCTACTCT ABI1 GCCGTTGTTTGTTCATCTC ACTGTATCACCTTGCCTCC ERF1 GCTCTTAACGTCGGATGGTC AGCCAAACCCTAGCTCCATT COI1 CAGCAGCCCATTGTTTCTTAC TACTGGCCAAGTACTTCCAATC ACTIN CGGTGCCCACTTTCCGATCT TCCTCACCGTCAGCCATTTT VIGS TRV:SlN-like CCGGAATTCCAATTCCGTCCTCTATGTCTC CCGCTCGAGGTTACCATCGAGAATCAATC