TaqMan探针法实时荧光定量PCR检测西瓜潜隐病毒

赵立群,邱艳红,张晓飞,刘慧,杨静静,张建,张海军,徐秀兰,温常龙

The Detection of Citrullus lanatus Cryptic Virus Using TaqMan-qPCR Method

ZHAO LiQun,QIU YanHong,ZHANG XiaoFei,LIU Hui,YANG JingJing,ZHANG Jian,ZHANG HaiJun,XU XiuLan,WEN ChangLong

图6 主要葫芦科作物种苗携带CiLCV的TaqMan-qPCR 检测（A）和RT-PCR检测（B） A：红色曲线表示阳性对照样品扩增结果,蓝色曲线表示12份待测样品扩增结果,最左侧蓝色曲线表示感病样品扩增结果The red curves indicate the amplification results of positive control samples, the blue curves indicate the amplification results of the 12 test samples, and the left blue curve presents the positive sample。B：CK+：阳性样品Positive control;M：DL2000 marker;1—12：样品1—12的PCR扩增产物The PCR results for sample 1-12;13：阴性对照Negative control

Fig. 6 The detection of CiLCV in main cucurbits crop seedlings with TaqMan-qPCR (A) and RT-PCR (B)