TaqMan探针法实时荧光定量PCR检测西瓜潜隐病毒

赵立群,邱艳红,张晓飞,刘慧,杨静静,张建,张海军,徐秀兰,温常龙

The Detection of Citrullus lanatus Cryptic Virus Using TaqMan-qPCR Method

ZHAO LiQun,QIU YanHong,ZHANG XiaoFei,LIU Hui,YANG JingJing,ZHANG Jian,ZHANG HaiJun,XU XiuLan,WEN ChangLong

图3 两对检测 CiLCV的探针特异性（A）和灵敏度（B）分析 蓝色和红色曲线分别对应CiLCV的探针1和探针2的扩增结果,绿色曲线代表其他8种病毒扩增曲线

Fig. 3 Analysis of specificity (A) and sensitivity (B) of detecting CiLCV with two sets of probes The blue and red curves indicate the amplification results of primer set 1 and primer set 2 for CiLCV, respectively, while the green curves indicate the amplification results of the other eight viruses