TaqMan探针法实时荧光定量PCR检测西瓜潜隐病毒

赵立群,邱艳红,张晓飞,刘慧,杨静静,张建,张海军,徐秀兰,温常龙

The Detection of Citrullus lanatus Cryptic Virus Using TaqMan-qPCR Method

ZHAO LiQun,QIU YanHong,ZHANG XiaoFei,LIU Hui,YANG JingJing,ZHANG Jian,ZHANG HaiJun,XU XiuLan,WEN ChangLong

表2 CiLCV检测引物序列

Table 2 The primer sequences for CiLCV detection

引物名称 Primer name 序列 Sequence (5′-3′) 目的 Purpose CiLCV-R1-F AGAATTTTCCCCCAGTCAAC CiLCV-dsRNA1全长克隆 Full length cloning of CiLCV-dsRNA1 CiLCV-R1-R AGAAGGTAAGGGTTAAATAAC CiLCV-R2-F AGAATTTTCCCCCAGTCAAC CiLCV-dsRNA2全长克隆 Full length cloning of CiLCV-dsRNA2 CiLCV-R2-R AGAAGGTAAGGGTTAAATAAC CiCLV-588-F TCCAGACGTTGGCTACACAC RT-PCR检测CiLCV CiLCV detection using RT-PCR CiCLV-588-R ATTGCGAACCTCTCAGGTGG DCiLCV-F1 AGAGGCACAGATGATGGATTTGG TaqMan-qPCR检测CiLCV CiLCV detection using TaqMan-qPCR DCiLCV-P1 AGCAGACCTTGAATTGATTACGGGTTC DCiLCV-R1 CATCCAGAAAGGCACTGCTCAT DCiLCV-F2 TGCCATTGAGACTGCTGTTCC TaqMan-qPCR检测CiLCV CiLCV detection using TaqMan-qPCR DCiLCV-P2 AGTACGACATGTATGGGGTCGCGC DCiLCV-R2 TCTGGGTACACTAACTGTCGGATC