甘薯丝裂原活化蛋白激酶MPK6对低温胁迫的响应

靳容,刘明,赵鹏,张强强,张爱君,唐忠厚

IbMKP6, A Mitogen-Activated Protein Kinase, Confers Low Temperature Tolerance in Sweetpotato

JIN Rong,LIU Ming,ZHAO Peng,ZHANG QiangQiang,ZHANG AiJun,TANG ZhongHou

图1 IbMPK6过表达甘薯转基因株系的建立和筛选 A：IbMPK6过表达载体结构示意图。B：IbMPK6过表达株系PCR鉴定。M：Marker;P：质粒;WT：非转基因对照植株,L1—L12：转基因株系。C：qRT-PCR检测筛选IbMPK6过表达株系。不同小写字母表示在P<0.01水平差异显著。红色边框内表示IbMPK6过表达最高的3个株系L3、L8和L11

Fig. 1 Generation of IbMPK6-overexpression transgenic sweetpotato plants A: Schematic diagram of IbMPK6-overexprssing constructs. B: Genomic DNA PCR analysis of IbMPK6-overexpressing transgenic sweetpotato lines. M: Marker; P: Plasmid; WT: Wide type; L1-L12: Transgenic plants. C: qRT-PCR analysis of IbMPK6-overexpressing transgenic sweetpotato lines. Lower-case letters indicated significantly different at P<0.01. The three lines (L3, L8 and L11) with the highest expression level of IbMPK6 were marked with red border