rhPA/GH双转基因兔的制备及rhPA表达检测
宋绍征1(
),于康英1,张婷2,陆睿2,潘生强1,成勇2(),周鸣鸣1()
),于康英1,张婷2,陆睿2,潘生强1,成勇2(),周鸣鸣1()
Preparation and Expression of rhPA/GH Double Transgenic Rabbits
SONG ShaoZheng1(),YU KangYing1,ZHANG Ting2,LU Rui2,PAN ShengQiang1,CHENG Yong2(),ZHOU MingMing1()
),YU KangYing1,ZHANG Ting2,LU Rui2,PAN ShengQiang1,CHENG Yong2(),ZHOU MingMing1()
图5. GH的PCR整合检测结果
1：正常非转基因兔（阴性对照）;2：K06-1转基因兔;3：K06-2转基因兔;4：K10-1转基因兔;5：K17-1转基因兔;6：rhPA单基因整合兔（K06）;7：PCL25/GH质粒（阳性对照）;M：DL2000 Marker
Fig. 5. Detection of GH by PCR
1: Normal non-transgenic rabbit (negative control); 2: K06-1 transgenic rabbit; 3: K06-2 transgenic rabbit; 4: K10-1 transgenic rabbit; 5: K17-1 transgenic rabbit; 6: rhPA monogenic integrated rabbit (K06); 7: PCL25/GH plasmid (positive control); M: DL2000 Marker
