rhPA/GH双转基因兔的制备及rhPA表达检测

宋绍征,于康英,张婷,陆睿,潘生强,成勇,周鸣鸣

Preparation and Expression of rhPA/GH Double Transgenic Rabbits

SONG ShaoZheng,YU KangYing,ZHANG Ting,LU Rui,PAN ShengQiang,CHENG Yong,ZHOU MingMing

表1 PCR扩增引物序列

Table 1 The primers sequences of PCR

引物名称 Primer name 引物序列 Primer sequence (5°-3°) gGH-F CCGCTCGAGCGGATGATGGCTGCAGGCCCCCGGA gGH-R CCGCTCGAGCGGCTAGAAGGCACAGCTGGCCTCCCCG CMV/tPA-F CGTGGATAGCGGTTTGA CMV/tPA-R GAGCCCTCCTTTGATGC