CRISPR/Cas9介导的绵羊示踪脐带间充质干细胞系的建立
李松美(
),仇雨歌,陈胜男,王晓萌,王春生()
),仇雨歌,陈胜男,王晓萌,王春生()
CRISPR/Cas9 Mediated Exogenous Gene Knock-in at ROSA26 Locus in Sheep Umbilical Cord Mesenchymal Stem Cells
LI SongMei(),QIU YuGe,CHEN ShengNan,WANG XiaoMeng,WANG ChunSheng()
),QIU YuGe,CHEN ShengNan,WANG XiaoMeng,WANG ChunSheng()
图3. T7E1酶切鉴定
1：Trans2K DNA Marker;2：野生型PCR产物;3：野生型T7E1酶切产物;4：突变型T7E1酶切产物
Fig. 3. T7E1 digestion identification
1: Trans2K DNA Marker; 2: Wild type PCR product; 3: Wild type T7E1 digestion product; 4: Mutated T7E1 digestion product
