CRISPR/Cas9介导的绵羊示踪脐带间充质干细胞系的建立
李松美(
),仇雨歌,陈胜男,王晓萌,王春生()
),仇雨歌,陈胜男,王晓萌,王春生()
CRISPR/Cas9 Mediated Exogenous Gene Knock-in at ROSA26 Locus in Sheep Umbilical Cord Mesenchymal Stem Cells
LI SongMei(),QIU YuGe,CHEN ShengNan,WANG XiaoMeng,WANG ChunSheng()
),QIU YuGe,CHEN ShengNan,WANG XiaoMeng,WANG ChunSheng()
图2. 转染效率
A：sUMSCs;B：转染后sUMSCs;C：对照组sUMSCs（50×）
（A：在sUMSCs细胞系中转染空载作为阳性对照;B、C：在sUMSCs细胞系中trim away技术,转入带有GFP的sROSA26-sgRNA-1/2/3载体,转染48h后的亮视野和暗视野,根据观察到的荧光强度确定转染效率）
Fig. 2. Transfection efficiency
A：sUMSCs;B：sUMSCs After transfecion;C：Control group sUMSCs（50×）
(A: the sUMSCs cell line after transfecting empty plasmid as a positive control; B, C:Transfer sROSA26-sgRN-1/2/3 vectors with GFP into sUMSCs cell lines using Trim Away technique, capture the bright and dark field after transfection for 48h, and the transfection efficiency was determined according to the observed fluorescence intensity.)
