CRISPR/Cas9介导的绵羊示踪脐带间充质干细胞系的建立
李松美(
),仇雨歌,陈胜男,王晓萌,王春生()
),仇雨歌,陈胜男,王晓萌,王春生()
CRISPR/Cas9 Mediated Exogenous Gene Knock-in at ROSA26 Locus in Sheep Umbilical Cord Mesenchymal Stem Cells
LI SongMei(),QIU YuGe,CHEN ShengNan,WANG XiaoMeng,WANG ChunSheng()
),QIU YuGe,CHEN ShengNan,WANG XiaoMeng,WANG ChunSheng()
图1. sROSA26-sgRNA-1 BbsⅠ酶切鉴定
1：Trans8k DNA Marker;2：PX330质粒;3：重组质粒BbsⅠ酶切鉴定
Fig. 1. The BbsⅠenzyme identification of sROSA26-sgRNA-1
1: Trans8k DNA Marker; 2: Plasmid PX330; 3: Enzyme identification of restructuring plasmid
