CRISPR/Cas9介导的绵羊示踪脐带间充质干细胞系的建立
李松美(
),仇雨歌,陈胜男,王晓萌,王春生()
),仇雨歌,陈胜男,王晓萌,王春生()
CRISPR/Cas9 Mediated Exogenous Gene Knock-in at ROSA26 Locus in Sheep Umbilical Cord Mesenchymal Stem Cells
LI SongMei(),QIU YuGe,CHEN ShengNan,WANG XiaoMeng,WANG ChunSheng()
),QIU YuGe,CHEN ShengNan,WANG XiaoMeng,WANG ChunSheng()
图17. 同源重组下游PCR鉴定
1：D2000 DNA Marker;2：同源重组下游PCR产物;3：野生型PCR产物
Fig. 17. Downstream PCR identification of homologous recombination
1: D2000 DNA Marker; 2: Homologous recombination downstream PCR product; 3: Wild type PCR product
