CRISPR/Cas9介导的绵羊示踪脐带间充质干细胞系的建立
李松美(
),仇雨歌,陈胜男,王晓萌,王春生()
),仇雨歌,陈胜男,王晓萌,王春生()
CRISPR/Cas9 Mediated Exogenous Gene Knock-in at ROSA26 Locus in Sheep Umbilical Cord Mesenchymal Stem Cells
LI SongMei(),QIU YuGe,CHEN ShengNan,WANG XiaoMeng,WANG ChunSheng()
),QIU YuGe,CHEN ShengNan,WANG XiaoMeng,WANG ChunSheng()
图15. 阳性细胞扩大培养
A：嘌呤霉素筛选16d后sUMSCs（明视野50×）;B：嘌呤霉素筛选16d后sUMSCs（暗视野50×）。
（将筛选后的阳性克隆扩大培养以获得足够的细胞数进行后续实验）
Fig. 15. Positive cells were further cultured
A: After puromycin screening for 16d sUMSCs（Bright field 50×）; B: After puromycin screening for 16d sUMSCs（Dark field 50×）
(Subculture the screened positive clones to obtain sufficient cell numbers for subsequent experimental studies)
